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Polymeric sorbents modified with gold and silver nanoparticles for solid-phase extraction of proteins followed by MALDI-TOF analysis

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Abstract

The authors describe four different kinds of sorbents for solid-phase extraction (SPE) and preconcentration of proteins from complex samples. All are based on the use of a poly(glycidyl-co-ethylene dimethacrylate) host monolith that was chemically functionalized by using two different ligands (ammonia and cysteamine). Gold nanoparticles (AuNPs) or silver NPs were then assembled to the amino or thiol groups. The resulting materials are shown to be viable stationary phases for use in SPE cartridges. The sorbents can selectively retain bovine serum albumin, and the thiol-modified sorbents containing AuNPs and AgNPs provide the highest recoveries (>90%) and satisfactory loading capacities (29.3 and 17.6 μg⋅mg−1 of sorbent, respectively). The applicability of these nanosorbents was demonstrated by preconcentrating viscotoxins from mistletoe extracts. The enriched fractions were subjected to MALDI-TOF analysis to underpin their selectivity.

Hybrid materials based on methacrylate polymers modified with gold or silver nanoparticles were used as sorbents for solid phase extraction and preconcentration of bovine serum albumin and mistletoe viscotoxins, this followed by MALDI-TOF analysis.

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Acknowledgements

This work was supported by project CTQ2014-52765-R (MINECO of Spain and FEDER) and PROMETEO/2016/145 (Generalitat Valenciana). M. V-B thanks the MEC for an FPU grant for PhD studies. The authors also thank Dr. Luz Valero Rustarazo and Dr. Salomé Laredo-Ortíz from the Proteomic and Atomic spectroscopy sections of the SCSIE (University of Valencia), for their help in MALDI-TOF-MS and Raman measurements, respectively.

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, E-46100, Burjassot, Valencia, Spain

    María Vergara-Barberán, María Jesús Lerma-García, Ernesto Francisco Simó-Alfonso & José Manuel Herrero-Martínez

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  1. María Vergara-Barberán
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  2. María Jesús Lerma-García
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  3. Ernesto Francisco Simó-Alfonso
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  4. José Manuel Herrero-Martínez
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Correspondence to María Vergara-Barberán or José Manuel Herrero-Martínez.

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Vergara-Barberán, M., Lerma-García, M.J., Simó-Alfonso, E.F. et al. Polymeric sorbents modified with gold and silver nanoparticles for solid-phase extraction of proteins followed by MALDI-TOF analysis. Microchim Acta 184, 1683–1690 (2017). https://doi.org/10.1007/s00604-017-2168-5

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