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The complete genome sequence of a double-stranded RNA mycovirus from Fusarium graminearum strain HN1

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Abstract

The complete nucleotide sequence of a double-stranded RNA (dsRNA) mycovirus, Fusarium graminearum dsRNA virus 5 (FgV5), was identified and characterized. The FgV5 genome comprises two dsRNA genome segments of 2030 bp and 1740 bp. FgV5 dsRNA1 contains a single open reading frame (ORF1), which is predicted to encode a protein of 613 amino acids (aa) with a molecular mass of 70.4 kDa and has a conserved RNA-dependent RNA polymerase (RdRp) motif. FgV5 dsRNA2 is predicted to contain two discontinuous ORFs (ORF2 and ORF3) that code for products of unknown function. Sequence comparisons showed that FgV5 has the highest aa sequence identities to Fusarium graminearum virus 4 (FgV4) (83.01% for ORF1, 78.70% for ORF2, and 76.27% for ORF3), suggesting that FgV5 and FgV4 should be regarded as members of different species. Phylogenetic analysis indicated that FgV5 belongs to a taxonomically unassigned dsRNA mycovirus group that is related to the families Amalgaviridae and Partitiviridae. Here, we propose that FgV5 and related viruses are members of a yet to be named and formally recognized new family.

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References

  1. Anagnostakis SL (1982) Biological control of chestnut blight. Science 215:466–471

    Article  CAS  PubMed  Google Scholar 

  2. Bruenn JA (1993) A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses. Nucleic Acids Res 21:5667–5669

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Campo S, Gilbert KB, Carrington JC (2016) Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum. PLoS Pathog 12:e1005640

    Article  PubMed  PubMed Central  Google Scholar 

  4. Chiba S, Salaipeth L, Lin YH, Sasaki A, Kanematsu S, Suzuki N (2009) A novel bipartite double-stranded RNA Mycovirus from the white root rot Fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 83:12801–12812

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Cho WK, Lee KM, Yu J, Son M, Kim KH (2013) Insight into mycoviruses infecting Fusarium species. Adv Virus Res 86:273–288

    Article  CAS  PubMed  Google Scholar 

  6. Chu YM, Jeon JJ, Yea SJ, Kim YH, Yun SH, Lee YW, Kim KH (2002) Double-stranded RNA mycovirus from Fusarium graminearum. Appl Environ Microbiol 68:2529–2534

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Chu YM, Lim WS, Yea SJ, Cho JD, Lee YW, Kim KH (2004) Complexity of dsRNA mycovirus isolated from Fusarium graminearum. Virus Genes 28:135–143

    Article  CAS  PubMed  Google Scholar 

  8. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    Article  CAS  PubMed  Google Scholar 

  9. Goswami RS, Kistler HC (2004) Heading for disaster: Fusarium graminearum on cereal crops. Mol Plant Pathol 5:515–525

    Article  CAS  PubMed  Google Scholar 

  10. Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe. Annu Rev Phytopathol 32:581–599

    Article  Google Scholar 

  11. Jiang Y, Zhang T, Luo C, Jiang D, Li G, Li Q, Hsiang T, Huang J (2015) Prevalence and diversity of mycoviruses infecting the plant pathogen Ustilaginoidea virens. Virus Res 195:47–56

    Article  CAS  PubMed  Google Scholar 

  12. King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, UK

    Google Scholar 

  13. Koloniuk I, Hrabakova L, Petrzik K (2015) Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:1585–1588

    Article  CAS  PubMed  Google Scholar 

  14. Kondo H, Chiba S, Toyoda K, Suzuki N (2013) Evidence for negative-strand RNA virus infection in fungi. Virology 435:201–209

    Article  CAS  PubMed  Google Scholar 

  15. Kotta-Loizou I, Sipkova J, Coutts RH (2015) Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:873–875

    Article  CAS  PubMed  Google Scholar 

  16. Lin Y, Zhang H, Zhao C, Liu S, Guo L (2015) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol 160:577–580

    Article  CAS  PubMed  Google Scholar 

  17. Liu L, Xie J, Cheng J, Fu Y, Li G, Yi X, Jiang D (2014) Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses. Proc Natl Acad Sci USA 111:12205–12210

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Marquez LM, Redman RS, Rodriguez RJ, Roossinck MJ (2007) A virus in a fungus in a plant: three-way symbiosis required for thermal tolerance. Science 315:513–515

    Article  CAS  PubMed  Google Scholar 

  19. Nerva L, Ciuffo M, Vallino M, Margaria P, Varese GC, Gnavi G, Turina M (2016) Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi. Virus Res 219:22–38

    Article  CAS  PubMed  Google Scholar 

  20. Nibert ML, Ghabrial SA, Maiss E, Lesker T (eds) (2013) A taxonomic proposal of the International Committee on Taxonomy of Viruses: Reorganization of family Partitiviridae to contain 4 new genera of plant and/or fungal viruses, with elimination of 3 current genera and creation of 16 new species

  21. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642

    Article  CAS  PubMed  Google Scholar 

  22. Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128

    Article  CAS  PubMed  Google Scholar 

  23. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Urayama S, Kato S, Suzuki Y, Aoki N, Le MT, Arie T, Teraoka T, Fukuhara T, Moriyama H (2010) Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae. J Gen Virol 91:3085–3094

    Article  CAS  PubMed  Google Scholar 

  25. Vainio EJ, Muller MM, Korhonen K, Piri T, Hantula J (2015) Viruses accumulate in aging infection centers of a fungal forest pathogen. ISME J 9:497–507

    Article  CAS  PubMed  Google Scholar 

  26. Valverde R, Nameth S, Jordan RL (1990) Analysis of double-stranded RNA for plant virus diagnosis. Plant Dis 74:255–258

    Article  Google Scholar 

  27. Wang S, Kondo H, Liu L, Guo L, Qiu D (2013) A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. Virus Res 174:69–77

    Article  CAS  PubMed  Google Scholar 

  28. Xie J, Wei D, Jiang D, Fu Y, Li G, Ghabrial S, Peng Y (2006) Characterization of debilitation-associated mycovirus infecting the plant-pathogenic fungus Sclerotinia sclerotiorum. J Gen Virol 87:241–249

    Article  CAS  PubMed  Google Scholar 

  29. Xie J, Xiao X, Fu Y, Liu H, Cheng J, Ghabrial SA, Li G, Jiang D (2011) A novel mycovirus closely related to hypoviruses that infects the plant pathogenic fungus Sclerotinia sclerotiorum. Virology 418:49–56

    Article  CAS  PubMed  Google Scholar 

  30. Yu J, Kwon SJ, Lee KM, Son M, Kim KH (2009) Complete nucleotide sequence of double-stranded RNA viruses from Fusarium graminearum strain DK3. Arch Virol 154:1855–1858

    Article  CAS  PubMed  Google Scholar 

  31. Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Zhang R, Liu S, Chiba S, Kondo H, Kanematsu S, Suzuki N (2014) A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses. Front Microbiol 5:360

    PubMed  PubMed Central  Google Scholar 

  33. Zheng L, Liu H, Zhang M, Cao X, Zhou E (2013) The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275. Arch Virol 158:1609–1612

    Article  CAS  PubMed  Google Scholar 

  34. Zhu HJ, Chen D, Zhong J, Zhang SY, Gao BD (2015) A novel mycovirus identified from the rice false smut fungus Ustilaginoidea virens. Virus Genes 51:159–162

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Lihua Guo.

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This study was funded by Science and Technology Plan Project of Beijing (No. D151100003915003) and the National Natural Science Foundation of China (31171818).

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All authors declare that they have no conflicts of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Wang, L., Wang, S., Yang, X. et al. The complete genome sequence of a double-stranded RNA mycovirus from Fusarium graminearum strain HN1. Arch Virol 162, 2119–2124 (2017). https://doi.org/10.1007/s00705-017-3317-1

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  • DOI: https://doi.org/10.1007/s00705-017-3317-1

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