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Structure and phase transitions of DMPC multilamellar vesicles in the presence of Ca2+ ions

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Abstract

Results obtained via small-angle neutron scattering studies of the influence of calcium ions on the structure and phase transitions of phospholipid membranes are presented. The main phase transition temperature of 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (1 wt %) multilamellar vesicles is demonstrated to increase by more than 1°C even when the calcium-ion content of the solution is low (0.1 mM). Detailed analysis of the multilamellar vesicles transition between “bound” and “unbound” state indicates the continuous character of the investigated process in both liquid and gel phases. The critical Ca2+ ion concentrations which initiate the destruction of the multilamellar structures and the formation of unilamellar vesicles are found to be ~0.3 mM in the gel and ~0.4–0.5 mM in the liquid-crystal phases during heating and ~0.5 mM in the phases under study upon cooling.

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Authors and Affiliations

  1. Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Moscow oblast, 141980, Russia

    Yu. E. Gorshkova, A. I. Kuklin & V. I. Gordeliy

  2. Laboratory for Advanced Studies of Membrane Proteins, Moscow Institute of Physics and Technology (MIPT), Dolgoprudny, Moscow oblast, 141700, Russia

    A. I. Kuklin & V. I. Gordeliy

  3. Institute of Structural Biology, Grenoble, 38044, France

    V. I. Gordeliy

  4. Institute of Structural Biology, Yulich, 52425, Germany

    V. I. Gordeliy

Authors
  1. Yu. E. Gorshkova
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  2. A. I. Kuklin
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  3. V. I. Gordeliy
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Correspondence to Yu. E. Gorshkova.

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Original Russian Text © Yu.E. Gorshkova, A.I. Kuklin, V.I. Gordeliy, 2016, published in Poverkhnost’, 2016, No. 11, pp. 34–45.

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Gorshkova, Y.E., Kuklin, A.I. & Gordeliy, V.I. Structure and phase transitions of DMPC multilamellar vesicles in the presence of Ca2+ ions. J. Surf. Investig. 11, 27–37 (2017). https://doi.org/10.1134/S1027451016050499

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  • DOI: https://doi.org/10.1134/S1027451016050499

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