biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:685-692, 2017 | DOI: 10.1007/s10535-017-0735-6

BrEXL6, a GDSL lipase gene of Brassica rapa, functions in pollen development

R. Ji1, H. Wang1, X. Xin1, S. Peng1, Y. Hur2, Z. Li3, H. Feng1,*
1 Department of Horticulture, Shenyang Agricultural University, Shenyang, P.R. China
2 Department of Biology, Chungnam National University, Daejeon, South Korea
3 Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, P.R. China

Multiple allele-inherited male sterility has been widely used by breeders of Brassica rapa L. ssp. pekinensis, but the molecular mechanisms of male sterility are not yet clear. In this study, we isolated the full-length cDNA of a new gene (not included in the Brassica database). This gene, comprising 1 054 bp, encodes a 39.99 kDa protein with a Gly-Asp- Ser-Leu (GDSL)-lipase domain that is a member of the lipolytic protein GDSL family. The sequence of candidate gene is the most similar to extracellular lipase 6 (EXL6) of Arabidopsis and was therefore designated BrEXL6 and submitted to NCBI (accession No. JX131630.1). Reverse transcription semi-quantitative PCR and Western blot analysis showed that BrEXL6 and its encoded protein were significantly more expressed in fertile buds than in sterile buds. Quantitative PCR and in situ hybridization showed that BrEXL6 was highly expressed in the anthers of fertile buds, especially anthers at the pollen-development stages, but only weakly expressed in other tissues and floral organs of fertile plants and whole sterile plants. These results suggest that BrEXL6 is a pollen development-related gene. The results of this study provide clues for understanding the mechanisms underlying multiple allele-inherited male sterility.

Keywords: Chinese cabbage; gene expression; in situ hybridization; male sterility; Western blot
Subjects: GDSL lipase gene; pollen development; in situ hybridization; male sterility; gene expression; Chinese cabbage

Received: August 8, 2015; Revised: January 21, 2017; Accepted: February 20, 2017; Published: December 1, 2017  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Ji, R., Wang, H., Xin, X., Peng, S., Hur, Y., Li, Z., & Feng, H. (2017). BrEXL6, a GDSL lipase gene of Brassica rapa, functions in pollen development. Biologia plantarum, 61(4), 685-692. doi: 10.1007/s10535-017-0735-6
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download filebpl-201704-0010_S1.pdf

    File size: 600.36 kB

    References

    1. Akoh, C.C., Lee, G.C., Liaw, Y.C., Huang, T.H., Shaw, J.F.: GDSL family of serine esterases/lipases. - Prog. Lipid Res. 43: 534-552, 2004. Go to original source...
    2. Chepyshko, H., Lai, C.P., Huang, L.M., Liu, J.H., Shaw, J.F.: Multifunctionality and diversity of GDSL esterase/lipase gene family in rice (Oryza sativa L. japonica) genome: new insights from bioinformatics analysis. - BMC Genomics 13: 309, 2012. Go to original source...
    3. Dong, X., Feng, H., Xu, M., Lee, J., Kim, Y.K., Lim, Y.P., Piao, Z., Park, Y.D., Ma, H., Hur, Y.: Comprehensive analysis of genic male sterility-related genes in Brassica rapa using a newly developed br300k oligomeric chip. - PLoS ONE 8: 274-274, 2013. Go to original source...
    4. Feng, H., Wei, Y., Ji, S., Jin, G., Jin, J., Dong, W.: Multiple allele model for genic male sterility in Chinese cabbage. - Acta Hort. 467: 133-142, 1996.
    5. Feng, H., Wei, Y., Zhang, S.: Inheritance of and utilization model for genic male sterility in Chinese cabbage (Brassica pekinensis Rupr.). - Acta Hort. 402: 133-140, 1995. Go to original source...
    6. Feng, H., Wei, P., Piao, Z., Liu, Z., Li, C., Wang, Y., Ji, R., Ji, S., Zou, T., Choi, S., Lim, Y.: SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.). - Theor. appl. Genet. 119: 333-339, 2009. Go to original source...
    7. Hu, W., Wang, Y., Bower, C., Ma, H.: Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis. - Plant mol. Biol. 53: 545-563, 2003. Go to original source...
    8. Ji, R., Song Q., Xin, X., Zhou, X., Feng, H.: Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP. - Genet. mol. Res. 10: 4073-4083, 2011a. Go to original source...
    9. Ji, R., Xin. X., Li, Z., Feng, H.: Construction and analysis of a suppression subtractive hybridization (SSH) library of genic multiple-allele inherited male-sterility in Chinese cabbage (Brassica campestris L. ssp. pekinensis). - Afr. J. Biotechnol. 10: 5296-5303, 2011b.
    10. Ji, R., Zhou, X., Song, Q., Xin, X., Feng, H.: [The relationship between bud length and microspore abortion in malesterility Brassica campestris L. ssp. pekinensis.] - Hubei agr. Sci. 50: 3552-3555, 2011c. [In Chin.]
    11. Li, C., Feng, H., Wei, P., Wang, L., Zhang, J., Yang, H., Jiang, N.: Directional transfer of the genic multiple allele inherited male sterile line in Chinese cabbage. - Afr. J. agr. Res. 4: 522-529, 2009.
    12. Ling, H., Zhao, J., Zuo, K., Qiu, C., Yao, H., Qin, J., Sun, X., Tang, K.: Isolation and expression analysis of a GDSL-like lipase gene from Brassica napus L. - J. Biochem. Mol. Biol. 39: 297-303, 2006. Go to original source...
    13. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. - Methods 25: 402-408, 2001. Go to original source...
    14. Mayfield, J.A., Fiebig, A., Johnstone, S.E., Preuss, D.: Gene families from the Arabidopsis thaliana pollen coat proteome. - Science 292: 2482-2485, 2001. Go to original source...
    15. Reumann, S., Ma, C., Lemke, S., Babujee, L.: AraPerox. A database of putative Arabidopsis proteins from plant peroxisomes. - Plant Physiol. 136: 2587-2608, 2004. Go to original source...
    16. Ten H.A., Breuil W.O., Wubben J.P. Visser J., Kan J.A.: Botrytis cinerea endopolygalacturonase genes are differentially expressed in various plant tissues. - Fungal Genet. Biol. 33: 97-105 2001. Go to original source...
    17. Upton, C., Buckley, J.T.: A new family of lipolytic enzymes? - Trends Biochem. Sci. 20: 178-179, 1995. Go to original source...
    18. Wang, L., Wei, P., Liu, Z., Li, C., Wang, Y., Ji, R., Feng, H.: SSR mapping of the Ms f, a multiple-allele male-fertility restorer gene in Chinese cabbage (Brassica campestris L. ssp. pekinensis). - Acta hort. sin. 37: 923-930, 2010.
    19. Wang, Y., Wang, X., Gu, M., Kang, H., Zeng, J., Fan, X., Sha, L., Zhang, H., Yu, K., Zhou, Y.: Cloning and characterization of four novel SnRK2 genes from Triticum polonicum. - Biol. Plant. 59: 211-219, 2015. Go to original source...
    20. Xu, J., Ding, Z., Vizcay-Barrena, G., Shi, J., Liang, W., Yuan, Z., Werck-Reichhart, D., Schreiber, L., Wilson, Z.A., Zhang, D.: Aborted microspores act as a master regulator of pollen wall formation in Arabidopsis. - Plant Cell 26: 1544-1556, 2014. Go to original source...
    21. Zeng, S., Ouyang, P., Mo, X., Wang, Y.: Characterization of genes coding phenylalanine ammonia lyase and chalcone synthase in four Pogostemon cablin cultivars. - Biol. Plant. 59: 298-304, 2015. Go to original source...

    Return to the content