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Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence

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Abstract

The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions.

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Correspondence to V. V. Evreinov.

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Original Russian Text © A.V. Gorshkov, M.L. Pridatchenko, T.Yu. Perlova, I.A. Tarasova, L.I. Levitsky, M.V. Gorshkov, V.V. Evreinov, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 12, pp. 1279–1289.

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Gorshkov, A.V., Pridatchenko, M.L., Perlova, T.Y. et al. Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence. J Anal Chem 71, 1188–1197 (2016). https://doi.org/10.1134/S106193481610004X

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  • DOI: https://doi.org/10.1134/S106193481610004X

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