Skip to main content
SpringerLink
Log in

Rapid detection of single nucleotide mutation in p53 gene based on gold nanoparticles decorated on graphene nanosheets

  • Regular Article
  • Published:
Journal of Chemical Sciences Aims and scope Submit manuscript

Abstract

A simple and highly sensitive electrochemical DNA sensor for the rapid detection of a specific DNA sequence related to the p53 gene is described. The structure and morphology of the synthesized graphene nanosheets and Au nanoparticles were characterized through transmission electron microscopy, UV–Vis spectroscopy and energy dispersion X-ray spectroscopy techniques. The new electrochemical biosensor was prepared by modifying a graphite screen printed electrode (SPE) with gold nanoparticles decorated on graphene (AuNPs/G) nanocomposite. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were employed to clarify the changes in charge transfer during the fabrication and utilization of the DNA biosensor. The DNA hybridization event was monitored by differential pulse voltammetry (DPV). Under optimal conditions, the decrease of the peak current had a linear relation with the logarithm of the concentration of target complementary DNA in the range 0.5 fM to 0.05 nM, with a detection limit of 0.36 ± 0.04 fM.

Rapid detection of DNA hybridization and single nucleotide mutation is reported by using gold nanoparticle-decorated, graphene nanosheets-modified screen printed electrode. Under optimal conditions, the peak current had a linear relation with the logarithm of the concentration of target complementary DNA in the range 0.5 fM to 0.05 nM, with a detection limit of 0.36 ± 0.04 fM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8

Similar content being viewed by others

References

  1. Guo Y, Chen J H and Chen G N 2013 Sens. Actuators, B 184 113

    Article  CAS  Google Scholar 

  2. Kim D, Kerman K, Saito M, Sathuluri R R, Endo T, Yamamura S, Kwon Y and Tamiya E 2007 Anal. Chem. 79 1855

    Article  CAS  Google Scholar 

  3. Patolsky F, Lichtenstein A and Willner I 2000 J. Am. Chem. Soc. 122 418

    Article  CAS  Google Scholar 

  4. Zhao X J, Dytioco R T and Tan W H 2003 J. Am. Chem. Soc. 125 11474

    Article  CAS  Google Scholar 

  5. He S J, Liu K K, Su S, Yan J, Mao X H, Wang D F, He Y L, Li J, Song S P and Fan C H 2012 Anal. Chem. 84 4622

    Article  CAS  Google Scholar 

  6. Silva M M S, Cavalcanti I T, Barroso M F, Sales M G F and Dutra R F 2010 J. Chem. Sci. 122 911

    Article  CAS  Google Scholar 

  7. Lao R J, Song S P W., H P W. L H, Zhang Z Z, He L and Fan C H 2005 Anal. Chem. 77 6475

    Article  CAS  Google Scholar 

  8. Erdem A and Ozsoz M 2001 Anal. Chim. Acta 437 107

    Article  CAS  Google Scholar 

  9. Hejazi M S, Raoof J B, Ojani R, Golabi S M and Hamidi-asl E 2010 Bioelectrochemistry 78 141

    Article  CAS  Google Scholar 

  10. Mousavi-Sani S Z, Raoof J B, Ojani R and Hamidi-Asl E 2013 J. Chin. Chem. Soc. 60 650

    Article  CAS  Google Scholar 

  11. Jin Y, Yao X, Liu Q and Li J 2007 Biosens. Bioelectron. 22 1126

    Article  CAS  Google Scholar 

  12. Zhang J, Song S P, Wang L H, Pan D and Fan C H 2007 Nature 2 2888

    CAS  Google Scholar 

  13. Wang J, Kawde A N and Musameh M 2003 Analyst 128 912

    Article  CAS  Google Scholar 

  14. Novoselov K S, Geim A K, Morozov S V, Jiang D, Zhang Y, Dubonos S V, Grigorieva I V and Firsov A A 2004 Science 306 666

    Article  CAS  Google Scholar 

  15. Huang X, Yin Z Y, Wu S X, Qi X Y, He Q Y, Zhang Q C, Yan Q Y, Boey F and Zhang H 2011 Small 7 1876

    Article  CAS  Google Scholar 

  16. Yang S, Li G, Hu M and Qu L 2014 J. Chem. Sci. 126 1021

    Article  CAS  Google Scholar 

  17. Jeena S E and Selvaraju T 2016 J. Chem. Sci. 128 357

    Article  CAS  Google Scholar 

  18. Vivekchand S R C, Rout C S, Subrahmanyam K S, Govindaraj A and Rao C N R 2008 J. Chem. Sci. 120 9

    Article  CAS  Google Scholar 

  19. Yin Z, Wu S, Zhou X, Huang X, Zhang Q, Boey F and Zhang H 2010 Small 6 307

    Article  CAS  Google Scholar 

  20. Sun X M, Liu Z, Welsher K, Robinson J T, Goodwin A, Zaric S and Dai H J 2008 Nano Res. 1 203

    Article  CAS  Google Scholar 

  21. Liu Z, Robinson J T, Sun X M and Dai H J 2008 J. Am. Chem. Soc. 130 10876

    Article  CAS  Google Scholar 

  22. Zhu L M, Luo L Q and Wang Z X 2012 Biosens. Bioelectron. 35 507

    Article  CAS  Google Scholar 

  23. Gao L N, Yue W B, Tao S S and Fan L Z 2013 Langmuir 29 957

    Article  CAS  Google Scholar 

  24. Xiao Y, Patolsky F, Katz E, Hainfeld J F and Willner I 2003 Science 299 1877

    Article  CAS  Google Scholar 

  25. Yola M L, Eren T and Atar N 2014 Electrochim. Acta 125 38

    Article  CAS  Google Scholar 

  26. Peng H P, Hua Y, Liu P, Deng Y N, Wang P, Chen W, Liu A L, Chen Y Z and Lin X H 2015 Sens. Actuators, B 207 269

    Article  CAS  Google Scholar 

  27. Read A P and Strachan T 1999 In Human Molecular Genetics 2nd ed. (New York: Wiley) Ch. 18

  28. Raoof J B, Ojani R, Golabi S M, Hamidi-Asl E and Hejazi M S 2011 Sens. Actuators, B 157 195

    Article  CAS  Google Scholar 

  29. Hamidi-Asl E, Raoof J B, Naghizadeh N, Sharifi S and Hejazi M S 2015 J. Chem. Sci. 127 1607

    Article  CAS  Google Scholar 

  30. Laschi S, Palchetti I, Marrazza G and Mascini M 2006 J. Electroanal. Chem. 593 211

    Article  CAS  Google Scholar 

  31. Hummers W S and Offernan R E 1958 J. Am. Chem. Soc. 80 1339

    Article  CAS  Google Scholar 

  32. Lei C X, Gong F C, Shen G L and Yu R Q 2003 Sens. Actuators, B 96 582

    Article  CAS  Google Scholar 

  33. Pournaghi-Azar M H, Hejazi M S and Alipour E 2006 Anal. Chim. Acta 570 144

    Article  CAS  Google Scholar 

  34. Pournaghi-Azar M H, Hejazi M S and Alipour E 2007 Electroanalysis 19 466

    Article  CAS  Google Scholar 

  35. Chen Q, Zhang L and Chen G 2012 Anal. Chem. 84 171

    Article  CAS  Google Scholar 

  36. Wang S, Yu D, Dai L, Chang D W and Baek J B 2011 ACS Nano 5 6202

    Article  CAS  Google Scholar 

  37. Brown K R, Walter D G and Natan M 2000 J. Chem. Mater. 12 306

    Article  CAS  Google Scholar 

  38. Yang T, Guan Q, Guo X H, Meng L, Du M and Jiao K 2013 Anal. Chem. 85 1358

    Article  CAS  Google Scholar 

  39. Yang L J, Li Y B and Erf G F 2004 Anal. Chem. 76 1107

    Article  CAS  Google Scholar 

  40. Wang Z J, Zhang J, Chen P, Zhou X Z, Yang Y, Wu S X, Niu L, Han Y, Wang L H, Chen P, Boey F, Zhang Q, Liedberg B and Zhang H 2011 Biosens. Bioelectron. 26 3881

    Article  CAS  Google Scholar 

  41. Wang X, Zhi L and Mullen K 2008 Nano Lett. 8 323

    Article  CAS  Google Scholar 

  42. Hu Y W, Li F H, Han D X, Wu T S, Zhang Q X, Niu L and Bao Y 2012 Anal. Chim. Acta 753 82

    Article  CAS  Google Scholar 

  43. Huang K J, Liu Y J, Wang H B, Gan T, Liu Y M and Wang L L 2014 Sens. Actuators, B 191 828

    Article  CAS  Google Scholar 

  44. Zhang Y and Jiang W 2012 Electrochim. Acta 71 239

    Article  CAS  Google Scholar 

  45. Huang J, Niu D J, Sun J Y, Han C H, Wu Z W, Li Y L and Xiong X Q 2011 Colloids Surf., B 82 543

    Article  CAS  Google Scholar 

  46. Han X W, Fang X, Shi A, Wang J and Zhang Y Z 2013 Anal. Biochem. 443 117

    Article  CAS  Google Scholar 

  47. Du Y, Guo S, Dong S and Wang E 2011 Biomaterials 32 8584

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran

    SEYEDEH ZEINAB MOUSAVISANI, JAHAN BAKHSH RAOOF, REZA OJANI & ROUDABEH VALIOLLAHI

Authors
  1. SEYEDEH ZEINAB MOUSAVISANI
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JAHAN BAKHSH RAOOF
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. REZA OJANI
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ROUDABEH VALIOLLAHI
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to JAHAN BAKHSH RAOOF.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

MOUSAVISANI, S.Z., RAOOF, J.B., OJANI, R. et al. Rapid detection of single nucleotide mutation in p53 gene based on gold nanoparticles decorated on graphene nanosheets. J Chem Sci 129, 131–139 (2017). https://doi.org/10.1007/s12039-016-1198-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12039-016-1198-6

Keywords

Search

Navigation