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A new spectrophotometric method for the micro determination of sulfuric acid esters in sulfatide fractions

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Summary

A new micro method has been developed for measuring sulfatide fractions. Sulfuric acid ester absorption is measured at 8.02 μm (μ) by infrared spectrophotometry, compared with that of a standard sulfatide, and corrected for phosphorus. General problems in measuring sulfatides are noted; procedural details, control studies, advantages, and limitations of the present method are discussed.

Zusammenfassung

Eine neue Mikromethode für die Bestimmung von Sulfatidfraktionen wird beschrieben. Die Absorption der Schwefelsäureester wird im Infrarot bei 8,02 μm gemessen, mit jener eines Sulfatidstandards verglichen und für Phosphor korrigiert. Allgemeine, mit der Bestimmung von Sulfatiden verbundene Probleme werden erwähnt; technische Einzelheiten, Kontroll-untersuchungen, Vorteile und Grenzen der beschriebenen Methode werden besprochen.

Résumé

Mise au point d'une nouvelle microméthode pour la détermination de fractions de sulfatides. L'absorption de l'ester sulfurique est mesurée à 8,02 μm (μ) par spectrophotométrie infra-rouge et comparée avec celle d'un sulfate standard puis corrigée du fait de la présence du phosphore. Les auteurs rappellent les problèmes généraux rencontrés dans la détermination des sulfatides. Les détails opératoires, les études de contrôle, les avantages et les limitations de la présente méthode sont d'autre part discutés.

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Authors and Affiliations

  1. Division of Neurology, University of Oregon Medical School, Portland, Oregon, USA

    Frank J. Witmer & James H. Austin

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  1. Frank J. Witmer
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  2. James H. Austin
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Witmer, F.J., Austin, J.H. A new spectrophotometric method for the micro determination of sulfuric acid esters in sulfatide fractions. Mikrochim Acta 48, 502–509 (1960). https://doi.org/10.1007/BF01215430

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  • DOI: https://doi.org/10.1007/BF01215430

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