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Análisis mutacional de los genes de la familiaTP53 en oligodendrogliomas

Mutation analyses of theTP53 gene family in oligodendroglial tumours

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Resumen

Introducción

La homología entreTP53/TP63/TP73 y la localización de este último en 1p36.33, zona frecuentemente delecionada en una gran variedad de tumores del sistema nervioso, sugiere que estos genes puedan estar implicados en la patogénesis de oligodendrogliomas.

Material y métodos

Para verificar esta hipótesis, hemos realizado un análisis mutacional mediante la técnica de PCR/SSCP y secuenciación de los genesTP53/TP63/TP73 en una serie de 41 oligodendrogliomas.

Resultados

No se encontró ninguna mutación en estos dos últimos genes, pero sí una enTP53 (518 T>C, Val173Ala). El resto de los cambios nucleotídicos identificados en las secuencias correspondieron a polimorfismos.

Conclusiones

Estos datos sugieren que la inactivación mutacional de los genes de la familia deTP53 no parece desempeñar un papel en el desarrollo neoplásico de oligodendrogliomas.

Abstract

Introduction

There is considerable homology amongTP53/TP63/TP73 genes. The last of these is located at 1p36.33; a chromosome region frequently deleted in a wide variety of tumours of the nervous system. This suggests that these genes could be involved in the development of oligodendrogliomas.

Material and methods

We performed a DNA mutation study using PCR/SSCP methods and sequencing of theTP53/TP63/TP73 genes in a series of 41 patients with oligodendrogliomas.

Results

We found one mutation (518 T→C; Val173Ala) in theTP53 gene and none in theTP63 andTP73 genes. The other nucleotide changes identified corresponded to polymorphisms.

Conclusions

These data suggest that mutations of theTP53 gene family do not appear to play a significant role in the pathology of oligodendrogliomas.

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Authors and Affiliations

  1. Laboratorio de Oncogenética Molecular Unidad de Investigación. Departamento de Cirugía Experimental, Hospital Universitario La Paz, Paseo Castellana, 261, 28046, Madrid, España

    María Eva Alonso Fernández, María Josefa Bello González, Cinthia Aniñoso Carbonero, Dolores Arjona Mayor, Pilar González Gómez, Jesús Lomas Huertas, Isabel López Marín & Juan Antonio Rey Herranz

  2. Departamento de Neurocirugía, Hospital del Río Hortega, Valladolid, España

    José María de Campos Gutiérrez

  3. Departamento de Neurocirugía, Clinica Puerta de Hierro, Madrid, España

    Jesús Vaquero Crespo

  4. Departamento de Neurocirugía, Hospital Universitario La Paz, Madrid, España

    Alberto Isla Guerrero

  5. Departamento de Anatomía Patológica, Hospital Universitario La Paz, Madrid, España

    Manuel Gutiérrez Molina

  6. Departamento de Anatomía Patológica, Fundación Jiménez Díaz, Madrid, España

    José Luis Sarasa Corral

Authors
  1. María Eva Alonso Fernández
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  2. María Josefa Bello González
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  3. Cinthia Aniñoso Carbonero
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  11. Manuel Gutiérrez Molina
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  13. Juan Antonio Rey Herranz
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Corresponding author

Correspondence to Juan Antonio Rey Herranz.

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Fernández, M.E.A., González, M.J.B., Carbonero, C.A. et al. Análisis mutacional de los genes de la familiaTP53 en oligodendrogliomas. Rev Oncol 6, 37–40 (2004). https://doi.org/10.1007/BF02710300

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  • DOI: https://doi.org/10.1007/BF02710300

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