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Asiatic Acid Inhibits Lipopolysaccharide-Induced Acute Lung Injury in Mice

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Abstract

Asiatic acid (AA), a major triterpene isolated from Centella asiatica (L.) Urban, is known to exert various pharmacological activities, including anti-inflammatory and antioxidant effects. The aim of this study was to evaluate the anti-inflammatory effects of AA on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and clarify the underlying mechanisms. Lung pathological changes were assessed by H&E staining. The myeloperoxidase (MPO) activity was detected by MPO assay. The levels of inflammatory cytokines were measured by ELISA. TLR4 and NF-kB expression was detected by Western blot analysis. AA obviously inhibited LPS-induced lung histopathological changes, MPO activity, and inflammatory cell numbers in bronchoalveolar lavage fluid (BALF). Treatment of AA also inhibited LPS-induced TNF-α, IL-6, and IL-1β production. Furthermore, Western blot analysis showed that AA inhibited LPS-induced TLR4 expression and NF-kB activation. In conclusion, AA inhibited LPS-induced ALI in mice by inhibiting inflammatory cytokine production, which is mediated via blocking of the TLR4/NF-kB signaling pathway.

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Authors and Affiliations

  1. Translational Medicine Center of Sepsis, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People’s Republic of China

    Zhiling Li, Xianzhong Xiao & Mingshi Yang

  2. Department of Critical Care Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People’s Republic of China

    Zhiling Li, Xianzhong Xiao & Mingshi Yang

  3. Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan, 410078, People’s Republic of China

    Zhiling Li & Xianzhong Xiao

Authors
  1. Zhiling Li
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  2. Xianzhong Xiao
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Correspondence to Xianzhong Xiao.

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Li, Z., Xiao, X. & Yang, M. Asiatic Acid Inhibits Lipopolysaccharide-Induced Acute Lung Injury in Mice. Inflammation 39, 1642–1648 (2016). https://doi.org/10.1007/s10753-016-0398-z

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  • DOI: https://doi.org/10.1007/s10753-016-0398-z

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