Abstract
The primary structures of the C and D subunits of sarcosine oxidase from Corynebacterium sp. U-96 were determined by sequencing the peptide fragments derived from their enzymatic digestions. The C and D subunits were shown to be composed of 199 and 92 residues, respectively. Each amino acid sequence showed a high homology with the sequence of the corresponding subunit from Corynebacterium sp. P-1. However, there were some differences between these two species, that is, four N-terminal residues were truncated in the C subunit, but six C-terminal residues were truncated in the D subunit. The D subunit contained three cysteine residues, but no disulfide bonds are in the subunit. Overall sequences of both subunit showed no homology with any other protein in the data base.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403–410.
Bairoch, A., and Apweiler, R. (1996) The SWISS-PROT protein sequence data bank and its new supplement TREMBL. Nucleic Acids Res. 24, 21–25.
Chlumsky, L. J., Zhang, L., and Jorns, M. S. (1995) Sequence analysis of sarcosine oxidase and nearby genes reveals homologies with key enzymes of folate one-carbon metabolism. J. Biol. Chem. 270, 18252–18259.
Chou, P. Y., and Fasman, G. D. (1978) Empirical predictions of protein conformation. Ann. Rev. Biochem. 47, 251–76.
Crestrield, A. M., Moore, S., and Stein, W. H. (1963) The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J. Biol. Chem. 238, 622–627.
Edman, P., and Begg, G. (1967) A Protein Sequenator. Eur. J. Biochem. 1, 80–91.
Hayashi, S., Nakamura, S., and Suzuki, M. (1980) Corynebacterium sarcosine oxidase: A unique enzyme having covalently-bound and noncovalently-bound flavins. Biochem. Biophys. Res. Commun. 96, 924–930.
Hewick, R. M., Hunkapiller, M. W., Hood, L. E., and Dreyer, W. J. (1981) A Gas-Liquid Solid Phase Peptide and Protein Sequenator. J. Biol. Chem. 256, 7990–7997.
Inouye, Y., Nishimura, M., Matsuda, Y., Hoshika, H., Iwasaki, H., Hujimura, K., Asano, K., and Nakamura, S. (1987) Purification and characterization of sarcosine oxidase of Streptomyces origin. Chem. Pharm. Bull. 35, 4194–4202.
Jorns, M. S. (1985) Properties and catalytic function of the two nonequivalent flavins in sarcosine oxidase. Biochemistry 24, 3189–3194.
Kawamura-Konishi, Y., and Suzuki, H. (1987) Kinetic studies on the reaction mechanism of sarcosine oxidase. Biochem. Biophys. Acta 915, 346–356.
Kim, J. M., Shimizu, S., and Yamada, H. (1987) Crystallization and characterization of sarcosine oxidase from Alcaligenes denitrificans subsp. denitrificans. Agric. Biol. Chem. 51, 1167–1168.
Koyama, Y., Yamamoto, Otake H., Suzuki, M., and Nakano, E. (1991) Cloning and expression of the sarcosine oxidase gene from Bacillus sp. NS-129 in Escherichia coli. Agric. Biol. Chem. 55, 1259–1263.
Matsuda, Y., Hoshika, H., Inouye, Y., Ikuta, S., Matsuura, K., and Nakamura, S. (1987) Purification and characterization of sarcosine oxidase of Bacillus origin. Chem. Pharm. Bull. (Tokyo) 35, 711–717.
Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262, 10035–10038.
Mori, N., Sano, M., Tani, Y., and Yamada, H. (1980) Purification and properties of sarcosine oxidase from Cylindrocarpon didymum M-1. Agric. Biol. Chem. 44, 1391–1397.
Ogushi, S., Nagao, K., Emi, S., Ando, M., and Tsuru, D. (1988) Sarcosine oxidase from Arthrobacter ureafaciens; Purification and some properties. Chem. Pharm. Bull. 36, 1445–1450.
Reuber, B. E., Karl, C., Reimann, S. A., Mihalik, S. J., and Dodt, G. (1997) Cloning and functional expression of a mammalian gene for a peroxisomal sarcosine oxidase. J. Biol. Chem. 272, 6766–6776.
Schägger, H., and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368–379.
Suzuki, H. (1994) Sarcosine oxidase: structure, function, and the application to creatinine determination. Amino Acids 7, 27–43.
Suzuki, H., and Kawamura-Konishi, Y. (1988) Presence of AMP binding sequence in subunit B of Corynebacterium sarcosine oxidase. Biochem. Int. 17, 577–583.
Suzuki, H., and Kawamura-Konishi, Y. (1991) Cysteine residues in the active site of Corynebacterium sarcosine oxidase. J. Biochem. 109, 909–917.
Suzuki, M. (1981) Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96. J. Biochem. 89, 599–607.
Wagner, M. A., and Jorns, M. S. (1997) Folate Utilization by Monomeric versus Heterotetrameric Sarcosine Oxidases. Arch. Biochem. Biophys. 342, 176–181.
Wierenga, R. K., Terpstra, P., and Hol, W. G. J. (1986) Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint. J. Mol. Biol. 187, 101–107.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mukouyama, E.B., Tatemukai, S., Morioka, K. et al. Determination of Amino Acid Sequences of Two Subunits in Sarcosine Oxidase from Corynebacterium sp. U-96. J Protein Chem 18, 747–752 (1999). https://doi.org/10.1023/A:1020625400518
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1020625400518