Abstract
The design of 1,3-diacylaminopropan-2-ols as CNS-directed carrier groups is based on their resemblance to endogenous lipids and the properties of pseudotriglyceride esters to facilitate the brain penetration of therapeutic agents. 2-[S-acetylthiorphan]-l,3-diacylaminopropan-2-ols, differing from the nature of 1,3-acyl chains, were synthesized and evaluated in vivo using the hot-plate jump test. The compounds exhibited naloxone reversible analgesic properties. The effects were superior to those of parent compounds thiorphan and S-acetylthiorphan. The palmitoyl derivative showed also activity at 0.8 mmol/kg after oral administration. Like acetorphan, a thiorphan prodrug, these compounds were poor substrates for brain enkephalinase, suggesting the release of the pharmacological active inhibitor at the site of action in the brain.
Similar content being viewed by others
REFERENCES
G. W. Goldstein, and A. L. Betz. The Blood-Brain Barrier. Sci. Am. 255:70–79 (1986).
J. B. M. M. Van Bree, A. G. De Boer, M. Danhof, and D. D. Breimer. Drug transport across the blood-brain barrier I. Anatomical and physiological aspects. Pharm. Weekbl [Sci] 14:305–310 (1992).
J. N. Jacob, V. E. Shashoua, A. Campbell, and R. J. Baldessarini. γ-Aminobutyric Acid Esters. 2. Synthesis, Brain Uptake, and Pharmacological Properties of Lipid Esters of γ-Aminobutyric Acid. J. Med. Chem. 28:106–110 (1985).
J. N. Jacob, J. W. Hesse, and V. E. Shashoua. γ-Aminobutyric Acid Esters. 2. Synthesis, Brain Uptake, and Pharmacological Properties of C-18 Glyceryl Lipid Esters of Gaba with Varying Degree of Unsaturation. J. Med. Chem. 30:1573–1576 (1987).
J. N. Jacob, J. W. Hesse, and V. E. Shashoua. Synthesis, Brain Uptake, and Pharmacological Properties of a Glyceryl Lipid Containing GABA and the GABA-T Inhibitor γ-Vinyl-GABA. J. Med. Chem. 33:733–736 (1990).
G. Y. Paris, and D. G. Cimon. Glycerides as Prodrugs. 4. Synthesis and Antiinflammatory Activity of 1,3-Dialkanoyl-2-arylalkanoyl-glycerides. Eur. J. Med. Chem. 17:193–195 (1982).
A. Garzon-Aburbeh, J. H. Poupaert, M. Claesen, P. A. Dumont, and G. Atassi. 1,3-Dipalmitoylglycerol Ester of Chlorambucil as a Lymphotropic, Orally Administrable Antineoplastic Agent. J. Med. Chem. 26:1200–1203 (1983).
A. Garzon-Aburbeh, J. H. Poupaert, M. Claesen, and P. A. Dumont. A Lymphotropic Prodrug of L-Dopa: Synthesis, Pharmacological Properties, and Pharmacokinetic Behaviour of 1,3-Dihexadecanoyl-2-[(S)-2-amino-3-(3,4-dihydroxyphenyl)pro-panoyl]propane-1,2,3-triol. J. Med. Chem. 29:687–691 (1986).
G. K. E. Scriba. Phenytoin-lipid conjugates: chemical, plasma esterase-and pancreatic lipase-mediated hydrolysis in vitro. Pharm. Res. 10:1181–1186 (1993).
J. C. Saraiva Goncalves. Propharmacomolécules sous forme de triacylglycérols: synthèse et évaluation des propriétés vectrices. Ph. D. thesis, School of Pharmacy, Catholic University of Louvain, Brussels, Belgium 1990.
F. Mergen, D. M. Lambert, J. C. Saraiva-Goncalves, J. H. Poupaert, and P. Dumont. Antiepileptic Activity of 1,3-Dihexadecanoylamino-2-valproyl-propan-2-ol, a Prodrug of Valproic Acid Endowed with Tropism for the CNS. J. Pharm. Pharmacol. 43:815–816 (1991).
D. M. Lambert, F. Mergen, J. H. Poupaert, and P. Dumont. Analgesic potency of S-acetylthiorphan after intravenous administration to mice. Eur. J. Pharmacol. 243:129–134.
B. P. Roques, F. Noble, V. Daugé, M. C. Fournié-Zaluski, and A. Beaumont. Neutral endopeptidase 24.11: structure, inhibition, and experimental and clinical pharmacology. Pharmacological Rev. 45:87–146 (1993).
R. Greenberg, and E. H. O'Keefe. Thiorphan Potentiation of Stress-Induced Analgesia in the Mouse. Life Sci. 31:1185–1188 (1982).
J.-M. Lecomte, J. Costentin, A. Vlaiculescu, P. Chaillet, H. Marcais-Collado, C. Llorens-Cortes, M. Leboyer, and J. C. Schwartz. Pharmacological Properties of Acetrophan, a Parenterally Active “Enkephalinase” Inhibitor. J. Pharmacol. Exp. Ther. 273:937–944 (1986).
B. Roques, J.-C. Schwartz, and J-M. Lecomte. (Société Civile Bioprojet, Paris, France) Aminoacid Derivatives and their Therapeutic Applications. European Patent 0 038 758-U.S. Patent 4000513009, 1981.
F. Mergen, D. M. Lambert, J. H. Poupaert, A. Bidaine, and P. Dumont. Synthesis of 1,3-Diacylaminopropan-2-ols and Corresponding 2-Acyl Derivatives as Amide Isosteres of Natural Lipids. Chem. Phys. Lipids 59:267–272 (1991).
A. M. Ondetti, M. E. Condon, J. Reid, E. F. Sabo, and H. S. Cheung. Design of Specific Inhibitors of Carboxypeptidases A and B. Biochemistry 18:1427–1430 (1979).
N. B. Eddy, and D. Leimbach. Synthetic Analgesics (II): Dithienylbutenyl-and Dithienylbutylamines. J. Pharmacol. Exp. Ther. 107:385–389 (1953).
M. C. Fournié-Zaluski, E. Lucas, G. Waskman, and B. P. Roques. Differences in the structural requirements for selective interaction with neutral metalloendopeptidase (enkephalinase) or angiotensin enzyme. Molecular investigation by use of new thiols inhibitors. Eur. J. Biochem. 139:267–274 (1984).
R. C. A. Frederickson, V. Bergers, and J. D. Edwards. Hyperalgesia induced by naloxone follows diurnal rhythm in responsivity to painful stimuli. Science, 198:756–758 (1977).
D. M. Lambert, L. Neuvens, F. Mergen, B. Gallez, J. H. Poupaert, J. Ghysel-Burton, J. M. Maloteaux, and P. Dumont. 1,3-Diacylaminopropan-2-ols and corresponding 2-acyl derivatives as drug carriers: unexpected pharmacological properties. J. Pharm. Pharmacol. 45:186–191 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lambert, D.M., Mergen, F., Berens, C.F. et al. Synthesis and Pharmacological Properties of 2-[S-Acetylthiorphan]-l,3-diacylaminopropan-2-ol Derivatives as Chimeric Lipid Drug Carriers Containing an Enkephalinase Inhibitor. Pharm Res 12, 187–191 (1995). https://doi.org/10.1023/A:1016214506667
Issue Date:
DOI: https://doi.org/10.1023/A:1016214506667