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Immune reactivity in cattle with ocular squamous cell carcinoma after intralesional BCG immunotherapy

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Summary

Lymphocyte stimulation with Con A and specific immune reactivity to BCG (antibody formation to BCG and DTH reaction to PPD) were determined in BCG-treated, surgically treated and untreated cows with ocular squamous cell carcinoma. In tumor-bearing cows the Con A-induced proliferation of lymphocytes was reduced when compared to healthy controls. This suppression consisted of a reduced blastogenic response to Con A of lymphocytes from tumor-bearing cows, and the presence of a factor in the sera of these animals, as these sera suppressed the blastogenic response of lymphocytes from healthy cows. BCG had only a minor influence on the suppressive activity. Antibodies to BCG were demonstrated in 50% of the BCG-treated animals. The formation of antibodies was not influenced by intradermal injection of PPD of Mycobacterium bovis. Absorption of a BCG antibody containing serum with BOSCC tumor extracts did not reveal the existence of cross reacting antigens between BCG and BOSCC. Pretherapeutic and posttherapeutic Con A reactivity could not be correlated with clinical response. Of the 30 BCG treated cows 29 developed a positive DTH reaction to PPD. Correlation between clinical response and immune reactivity was seen only with regard to the DTH reaction to PPD: this reaction remained positive for a longer period after treatment in animals with a favorable clinical outcome than in nonresponding animals.

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Authors and Affiliations

  1. Institute for Veterinary Surgery, State University Utrecht, Yalelaan 12, 3584 CM, Utrecht, The Netherlands

    Wim R. Klein

  2. Laboratory for Pathology, Rijksinstituut voor Volksgezondheid en Milieuhygiene (RIVM), P. O. Box 1, 3720 BA, Bilthoven, The Netherlands

    Peter A. Steerenberg, Wim H. de Jong & E. Joost Ruitenberg

  3. Department of Immunology, Faculty of Veterinary Medicine, State University Utrecht, Yalelaan 1, 3584 CL, Utrecht, The Netherlands

    Fred Poelma, Elly v. d. Wiel, Victor P. M. G. Rutten & E. Joost Ruitenberg

  4. Department of Pathology, Division of Clinical Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands

    Wim Misdorp

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  1. Wim R. Klein
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  2. Peter A. Steerenberg
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Additional information

Animals were maintained under the guidelines laid down by the Faculty of Veterinary Medicine, State University, Utrecht, The Netherlands

Grant recipient of the Koningin Wilhelmina Fonds (Netherlands Cancer Foundation)

Abbreviations used: BCG, Bacillus Calmette-Guerin; BOSCC, bovine ocular squamous cell carcinoma PBL peripheral blood leukocytes; PPD, purified protein derivative of Mycobacteria; DTH, delayed type hypersensitivity Con A, concanavalin A; PHA, phytohemagglutinin; PWM, pokeweed mitogen

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Klein, W.R., Steerenberg, P.A., Poelma, F. et al. Immune reactivity in cattle with ocular squamous cell carcinoma after intralesional BCG immunotherapy. Cancer Immunol Immunother 22, 87–94 (1986). https://doi.org/10.1007/BF00199120

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  • DOI: https://doi.org/10.1007/BF00199120

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