Zusammenfassung: | |
Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection. © 2016 Elsevier Ltd
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Lizenzbestimmungen: | Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2017 |
Schlagwörter (englisch): | 2D-IEF/SDS/PAGE, Frankliniella occidentalis, Innate immunity, Mass spectrometry, Proteomics, Tomato spotted wilt virus, actin, actin related protein 2, adenosylhomocysteinase, aldehyde reductase, aldose reductase related protein 1, alpha tubulin, cathepsin L, chitinase, cyclic AMP dependent protein kinase anchoring protein, dermcidin, DNA polymerase Rev1, enolase, fibrinogen, guanine nucleotide binding protein beta subunit, guanosine phosphate reductase, heat shock protein 70, heterochromatin protein 1, hornerin, hyaluronidase, inactive hyaluronidase B, initiation factor 3, initiation factor 5A, khellin, membrane protein, proton transporting adenosine triphosphate synthase, ribosome protein, ribosome protein L12, ubiquitin, unclassified drug, unindexed drug, valosin containing interacting protein 135, dermcidin, heat shock protein 70, insect protein, peptide, transcriptome, ubiquitin, Article, Frankliniella occidentalis, immune response, innate immunity, isoelectric focusing, mass spectrometry, nonhuman, nucleotide metabolism, pilot study, polyacrylamide gel electrophoresis, priority journal, protein analysis, protein expression, protein function, proteomics, Tomato spotted wilt virus, virus isolation, animal, arthropod vector, gene expression regulation, genetics, immunology, larva, lipid metabolism, metabolism, proteomics, Thysanoptera, Tospovirus, Animals, Arthropod Vectors, Gene Expression Regulation, HSP70 Heat-Shock Proteins, Immunity, Innate, Insect Proteins, Larva, Lipid Metabolism, Mass Spectrometry, Peptides, Proteomics, Thysanoptera, Tospovirus, Transcriptome, Ubiquitin |
Fachliche Zuordnung (DDC): | 610 | Medizin, Gesundheit |
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