Abstract
Phosphorus (P) is an essential macronutrient to all crops including rice and it plays a key role in various plant activities and development. Low availability of P in the soils negatively, influences rice crop growth and causes significant yield loss. In the present study, we characterized a set of 56 germplasm lines for their tolerance to low soil P by screening them at low soil P and optimum soil P levels along with low soil P tolerant and sensitive check varieties. These lines were genotyped for the presence/absence of tolerant allele with respect to the major low soil P tolerance QTL, Pup1, using a set of locus specific PCR-based markers, viz., K46-1, K46-2, K52 and K46CG-1. High genetic variability was observed for various traits associated with low soil P tolerance. The yield parameters from normal and low soil P conditions were used to calculate stress tolerance indices and classify the genotypes according to their tolerance level. Out of the total germplasm lines screened, 15 lines were found to be tolerant to low soil P condition based on the yield reduction in comparison to the tolerant check, but most of them harbored the complete or partial Pup1 locus. Interestingly, two tolerant germplasm lines, IC216831 and IC216903 were observed to be completely devoid of Pup1 and hence they can be explored for new loci underlying low soil P tolerance.
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References
Akinrinde EA, Gaizer T (2006) Differences in the performance and phosphorus-use efficiency of some tropical rice (oryza sativa l.) varieties. Pak J Nutr 5(3):206–211
Aluwihare C, Ishan M, Chamikara MDM, Weebadde CK, Sirisena DN, Samarasinghe WLG, Sooriyapathirana SDSS (2016) Characterization and selection of phosphorus deficiency tolerant rice genotypes in Sri Lanka. Rice Sci 23(4):184–195
Anila M, Mahadeva Swamy HK, Kale RR, Bhadana VP, Anantha MS, Brajendra Hajira SK, Balachiranjeevi CH, Ayyappa Dass M, Bhaskar S, Dilip T, Pranathi K, Kousik MBVN, Harika G, Swapnil K, Chaitra K, Laxmi Prasanna B, Punniakotti E, Sinha P, Rekha G, Abhilash Kumar V, Balachandran SM, Madhav MS, Giri A, Viraktamath BC, Sundaram RM (2018) Breeding lines of the Indian mega-rice variety, MTU 1010, possessing protein kinase OsPSTOL (Pup1), show better root system architecture and higher yield in soils with low phosphorus. Mol Breed 38:147
Ashraf A, El-Mohsen A, Abd El-Shafi MA, Gheith EMS, Suleiman HS (2015) Using different statistical procedures for evaluating drought tolerance indices of bread wheat genotypes. Adv Agric Biol 3(1):19–30
Assuero SG, Mollier A, Pellerin S (2004) The decrease in growth of phosphorus deficient maize leaves is related to a lower cell production. Plant, Cell Environ 27:887–895
Bouslama M, Schapaugh WT (1984) Stress tolerance in soybean. Part 1: evaluation of three screening techniques for heat and drought tolerance. Crop Sci 24:933–937
Cancellier EL, Brandao DR, Silva J, Santos MM, Fidelis RR (2012) Phosphorus use efficiency of upland rice cultivars on Cerrado soil. Ambience 8(2):307–318
Chankaew S, Monkham T, Pinta W, Sanitchon J, Kaewpradit W, Srinives P (2019) Screening tolerance to phosphorus deficiency and validation of phosphorus uptake 1 (Pup1) gene-linked markers in Thai indigenous upland rice germplasm. Agronomy 9:81. https://doi.org/10.3390/agronomy9020081
Chauhan YS, Johansen C, Venkataratnam N (1992) Effects of phosphorus deficiency on phenology and yield components of short-duration pigeonpea. Trop Agric 69(3):235–238
Chiangmai PN, Yodmingkhwan P (2011) Competition of root and shoot growth between cultivated rice (Oryza sativa L.) and common wild rice (Oryza rufipogon Griff.) grown under different phosphorus levels. Songklanakarin Journal of Science and Technology 33(6):685–692
Chin JH, Lu X, Haefele SM, Gamuyao R, Ismail A, Wissuwa M, Heuer S (2010) Development and application of gene-based markers for the major rice QTL phosphorus uptake 1. Theor Appl Genet 120:1073–1086
Chin JH, Gamuyao R, Dalid C, Bustamam M, Prasetiyono J, Moeljopawiro S, Wissuwa M, Heuer S (2011) Developing rice with high yield under phosphorus deficiency: Pup1 sequence to application1. Plant Physiol 156(3):1202–1216
Chithrameenal K, Alagarasan G, Raveendran M, Robin S, Meena S, Ramanathan A, Ramalingam J (2018) Genetic enhancement of phosphorus starvation tolerance through marker assisted introgression of OsPSTOL1 gene in rice genotypes harbouring bacterial blight and blast resistance. PLoS ONE 13(9):e0204144. https://doi.org/10.1371/journal.pone.0204144
Cordell D, White S (2011) Peak phosphorus: clarifying the key issues of a vigorous debate about long-term phosphorus security. Sustainability 3:2027–2049
Cordell D, Drangert JO, White S (2009) The story of phosphorus: global food security and food for thought. Glob Environ Chang 19:292–305
Fageria NK, Baligar VC (1997) Upland rice genotypes evaluation for phosphorus use efficiency. J Plant Nutr 20:499–509
Fageria NK, Wright RJ, Baligar VC (1988) Rice cultivar evaluation for phosphorus use efficiency. J Plant Nutr Soil Sci 111:105–109
Farshadfar E, Mohammadi R, Farshadfar M, Dabiri S (2013) Relationships and repeatability of drought tolerance indices in wheat-rye disomic addition lines. Aust J Crop Sci 7(1):130–138
Fernandez GCJ (1992) Effective selection criteria for assessing plant stress tolerance. In: Proceeding of the international. Symposium, on adaptation of vegetables and other food crops in temperature and water stress. Taiwan, pp 257–270
Fisher AT, Maurer R (1978) Drought resistance in spring wheat cultivars. I. Grain yield responses. Aust J Agric Res 29:897–912
Gamuyao R, Chin HJ, Tanaka JP, Pesaresi P, Catausan S, Dalid C, Loedin SI, Mendoza TME, Wissuwa M, Heuer S (2012) The protein kinase Pstol1 from traditional rice confers tolerance of phosphorus deficiency. Nature 88:535–541
Gavuzzi P, Rizza F, Palumbo M, Campaline RG, Ricciardi GL, Borghi B (1997) Evaluation of field and laboratory predictors of drought and heat tolerance in winter cereals. Can J Plant Sci 77:523–531
Golestani Araghi S, Assad MT (1998) Evaluation of four screening techniques for drought resistance and their relationship to yield reduction ratio in wheat. Euphytica 103:293–299
Heuer S, Gaxiola R, Schilling R, Herrera-Estrella L, Lopez-Arredondo D, Wissuwa M, Delhaize E, Rouached H (2017) Improving phosphorus use efficiency: a complex trait with emerging opportunities. Plant J 90:868–885
Kavanova M, Lattanzi FA, Grimoldi AA, Schnyder H (2006) Phosphorus deficiency decreases cell division and elongation in grass leaves. Plant Physiol 141:766–775
Khan U, Mohammad F (2016) Application of stress selection indices for assessment of nitrogen tolerance in wheat (Triticum aestivum L.). J Anim Plant Sci 26(1):201–210
Khush GS, Jena KK (2005) Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In: Advances in genetics, genomics and control of rice blast disease, pp 1–10
Kirk GJD (2004) The biogeochemistry of submerged soils. John, Chichester
Kirk GJD, George T, Courtois B, Senadhira D (1998) Opportunities to improve phosphorus efficiency and soil fertility in rainfed lowland and upland rice ecosystems. Field Crop Res 56:73–92
Koyama T, Chammek C, Snitwonge P (1973) Varietal differences of Thai rice in the resistance to phosphorus deficiency. Tech Bull Trop Agric Res Cent 4:32
Li Z, Xie Y, Dai A, Liu L (2009) Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice. J Genet Genom 36:173–183
Lopez-Arredondo DL, Leyva-Gonz´alez MA, Gonz´alez-Morales SI, Lopez-Bucio J, Herrera-Estrella L (2014) Phosphate nutrition: improving low-phosphate tolerance in crops. Annu Rev Plant Biol 65:95–123
Mollasadeghi V, Valizadeh M, Shahryariand RA, Imani A (2011) Evaluation of end drought tolerance of 12 wheat genotypes by stress in dices. Middle East J Sci Res 7(2):241–247
Neelam K, Thakur S, Neha, Yadav IS, Kumar K, Dhaliwal SS, Singh K (2017) Novel alleles of phosphorus-starvation tolerance 1 gene (PSTOL1) from Oryza rufipogon confers high phosphorus uptake efficiency. Front Plant Sci 8:509. https://doi.org/10.3389/fpls.2017.00509
Nord E, Lynch J (2008) Delayed reproduction in Arabidopsis thaliana improves fitness in soil with suboptimal P availability. Plant, Cell Environ 31:1432–1441
Perrier X, Jacquemoud-Collet JP (2006) Darwin software. http://darwin.cirad.fr/darwin. Accessed 3 Jun 2019
Pinheiro BS, Castro E, Guimarães CM (2006) Sustainability and profitability of aerobic rice production in Brazil. Field Crops Res 97:34–42
Raghothama KG, Karthikeyan AS (2005) Phosphate acquisition. Plant Soil 274:37–49
Rameeh V (2015) Nitrogen deficiency stress indices of seed yield in rapeseed (Brassica napus L.) genotypes. Cercet Agron Mold 158(1/161):89–96
Rodriguez D, Pomar MC, Goudriaan J (1998) Leaf primordia initiation, leaf emergence and tillering in wheat (Triticum aestivum L.) grown under low-phosphorus conditions. Plant Soil 202(1):149–157
Rosielle AA, Hamblin J (1981) Theoretical aspect of selection for yield in stress and non- stress environment. Crop Sci 21:943–946
Sarkar S, Yelne R, Chatterjee M, Das P, Debnath S, Chakraborty A, Mandal N, Bhattacharya K, Bhattacharyya S (2011) Screening for phosphorus (P) tolerance and validation of Pup-1 linked markers in indica rice. Indian J Genet 71(3):209–213
Shepherd KD, Cooper PJM, Allan AY, Drennan DSH, Keatinge JDH (1987) Growth, water-use and yield of barley in Mediterranean-type environments. J Agric Sci 108:365–378
Shimizu A, Yanagihara Kawasaki S, Ikehashi H (2004) Phosphorus deficiency induced root elongation and its QTL in rice (Oryza sativa L.). Theor Appl Genet 109(7):1361–1368
Singh S, Sengar RS, Kulshreshtha N, Datta D, Tomar RS, Rao VP, Garg D, Ojha A (2015) Assessment of multiple tolerance indices for salinity stress in bread wheat (Triticum aestivum L.). J Agric Sci 7(3):49–57
Swamy HKM, Anila M, Kale RR, Bhadana VP, Anantha MS, Brajendra P, Hajira SK, Balachiranjeevi CH, Prasanna BL, Pranathi K, Dilip T, Bhaskr S, Kumar AV, Kousik MBVN, Harika G, Swapnil K, Rekha G, Cheralu C, Shankar VG, Reddy NS, Kumar S, Balachnadran SM, Madhav MS, Kumar RM, Sundaram RM (2019) Phenotypic and molecular characterization of rice germplasm lines and identification of novel source for low soil phosphorus tolerance in rice. Euphytica 215:118. https://doi.org/10.1007/s10681-019-2443-0
Tanaka JP, Chin JH, Drame KN, Daid C, Heuer S, Wissuwa M (2014) A novel allele of the P-Starvation tolerance gene OsPSTOL1 from African rice (Oryza glaberrima Steud) and its distribution in the genus Oyza. Theor Appl Genet 127:1387–1398
Tian Z, Li J, He X, Jia X, Yang F, Wang Z (2017) Grain yield, dry weight and phosphorus accumulation and translocation in two rice (Oryza sativa L.) varieties as affected by salt-alkali and phosphorus. Sustainability 9:1461. https://doi.org/10.3390/su9081461
Tyagi W, Rai M, Dohling AK (2012) Haplotype analysis for Pup1 locus in rice genotypes of North-Eastern and Eastern India to identify suitable donors tolerant to low phosphorus. SABRAO J Breed Genet 44:398–405
Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing a non-renewable resource. New Phytol 157:423–447
Vejchasarn P, Lynch JP, Brown KM (2016) Genetic variability in phosphorus responses of rice root phenotypes. Rice 9:29. https://doi.org/10.1186/s12284-016-0102-9
Wang A, Chen F, Zhang F, Guohua M (2010) Two strategies for achieving higher yield under phosphorus deficiency in winter wheat grown in field conditions. Field Crops Res 118:36–42
Webeck E, Matsubae K, Nakajima K, Nansai K, Nagasaka T (2014) Analysis of phosphorus dependency in Asia. Sociotechnica 11:119–126
Wissuwa M (2005) Combining a modelling with a genetic approach in establishing associations between genetic and physiological effects in relation to phosphorus uptake. Plant Soil 269:57–68
Wissuwa M, Ae N (2001) Genotypic variation for tolerance to phosphorus deficiency in rice and the potential for its exploitation in rice improvement. Plant Breed 120:43–48
Wissuwa M, Yano M, Ae N (1998) Mapping of QTLs for phosphorus deficiency tolerance in rice (Oryza sativa L.). Theor Appl Genet 97:777–783
Wissuwa M, Wegner J, Ae N, Yano M (2002) Substitution mapping of Pup1: a major QTL increasing phosphorus uptake of rice from a phosphorus-deficient soil. Theor Appl Genet 105:890–897
Wissuwa M, Mazzola M, Picard C (2009) Novel approaches in plant breeding for rhizosphere-related traits. Plant Soil 321:409–430
Zheng K, Huang N, Bennett J, Khush GS (1995) PCR-based marker assisted selection in rice breeding. IRRI Discussion Paper Series No. 12. International Rice Research Institute, Manila, Philippines
Acknowledgements
The authors are thankful to the Director of ICAR-Indian Institute of Rice Research for contributing the required lab facilities and Institute of biotechnology PJTSAU, Telangana for providing the facilities and encouragement. The first author is also thankful to CSIR for providing financial support to carry out PhD programme under the scheme of CSIR-JRF.
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CSIR JRF (09/891(0004)/2015EMR-I, which has provided significant financial support for carrying out the present study.
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Kale, R.R., Anila, M., Mahadeva Swamy, H.K. et al. Morphological and molecular screening of rice germplasm lines for low soil P tolerance. J. Plant Biochem. Biotechnol. 30, 275–286 (2021). https://doi.org/10.1007/s13562-020-00586-5
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DOI: https://doi.org/10.1007/s13562-020-00586-5