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Introduction of a Proximal Stat5 Site in the Murine α-Lactalbumin Promoter Induces Prolactin Dependency In Vitro and Improves Expression Frequency In Vivo

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Abstract

In order to establish a possible correlation between in vitro prolactin induction and the transcriptional activity of mammary gene promoters in transgenic mice, a functional Stat5-binding site was created by means of site-directed mutagenesis at position −70 on a 560 bp murine α-lactalbumin promotor linked to a CAT reporter gene. Surprisingly, the wild-type promoter was constitutively active in vitro and could not be induced by prolactin. Introducing the proximal Stat5 site abolished this constitutive activity and resulted in prolactin dependence in both CHO-K1- and HC11-transfected cells. In transgenic mice, both the frequency of lines expressing the transgene and the prevalence of mid to late pregnancy expression were increased.

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Authors and Affiliations

  1. Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352, Jouy-en-Josas Cedex, France

    Solange Soulier, Laurence Lepourry, Marie-georges Stinnakre, Jacqueline Paly, Jean Djiane, Jean-claude Mercier & Jean-luc Vilotte

  2. AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand

    Brett Langley & Phil J. L'Huillier

Authors
  1. Solange Soulier
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  2. Laurence Lepourry
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  4. Brett Langley
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  5. Phil J. L'Huillier
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  6. Jacqueline Paly
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  7. Jean Djiane
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  8. Jean-claude Mercier
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  9. Jean-luc Vilotte
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Soulier, S., Lepourry, L., Stinnakre, Mg. et al. Introduction of a Proximal Stat5 Site in the Murine α-Lactalbumin Promoter Induces Prolactin Dependency In Vitro and Improves Expression Frequency In Vivo. Transgenic Res 8, 23–31 (1999). https://doi.org/10.1023/A:1008851802022

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