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72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

06.06. - 09.06.2021

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Evaluation of morphology and behaviour of microglia obtained from human glioma

Evaluation der Morphologie und des Verhaltens der Mikrogliazellen aus humanen Gliomen

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  • presenting/speaker Maryam Khaleghi Ghadiri - Münster University, Neurosurgery, Münster, Deutschland
  • Sepideh Ghasemi - Münster University, Neurosurgery, Münster, Deutschland
  • Zoé Labreze - Münster University, Neurosurgery, Münster, Deutschland
  • Siw Folkens - Münster University, Neurosurgery, Münster, Deutschland
  • Walter Stummer - Münster University, Neurosurgery, Münster, Deutschland
  • Ali Gorji - Münster University, Neurosurgery, Münster, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie. sine loco [digital], 06.-09.06.2021. Düsseldorf: German Medical Science GMS Publishing House; 2021. DocP075

doi: 10.3205/21dgnc363, urn:nbn:de:0183-21dgnc3632

Published: June 4, 2021

© 2021 Khaleghi Ghadiri et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Objective: Microglia, mono-nuclear cells distributed throughout the CNS, are key immune effector cells of the brain. The complex interactions between microglia and the other cell types in glioma play a crucial role in the development of novel treatments, presumably via the regulation of tumor progression and anti-tumor immune responses. This study was aimed to evaluate the morphology and behavior of microglia isolated from human glioma and compare these characteristics with microglia obtained from epileptic brain tissues.

Methods: The morphology, expression of the microglia-specific binding protein, migration ability, phagocytosis, apoptosis, and the expression of excitatory as well as inhibitory receptors were assessed.

Results: Our preliminary findings have shown a wide range of various morphologies of both activated- and non-activated microglia associated with strong spatiotemporal alterations in morphology over 4 weeks of cell culture. Furthermore, different forms of microglia exhibited different patterns of migration ability. Various patterns of the microglia-specific binding protein, as well as glutamate and GABA receptor expression, were identified in microglia with different morphologies. Microglia with different morphologies exerted different phagocytic properties. The behavior and morphology of microglia obtained from glioblastoma tissues were markedly different from those from epileptic brain tissues.

Conclusion: Our findings provide comprehensive information on the behavior of microglia in glioma, setting the basis for a more detailed classification and new insights for the designation of novel glioma treatment via targeting microglia.