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68th Annual Meeting of the German Society of Neurosurgery (DGNC)
7th Joint Meeting with the British Neurosurgical Society (SBNS)

German Society of Neurosurgery (DGNC)

14 - 17 May 2017, Magdeburg

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Parvovirus B19 infection in intervertebral disc

Meeting Abstract

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  • Andreas Reinke - Orthopädie, Unfallchirurgie und Wirbelsäulentherapie, Donauwörth, Deutschland
  • Miriam Sailer - Klinikum rechts der Isar, TU München, Neurochirurgie, München, Deutschland
  • Michael Behr - Klinikum rechts der Isar, TU München, Neurochirurgie, München, Deutschland
  • Bernhard Meyer - Klinikum rechts der Isar, TU München, Neurochirurgie, München, Deutschland
  • Jens Lehmberg - Klinikum rechts der Isar, TU München, Neurochirurgie, München, Deutschland

Deutsche Gesellschaft für Neurochirurgie. Society of British Neurological Surgeons. 68. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 7. Joint Meeting mit der Society of British Neurological Surgeons (SBNS). Magdeburg, 14.-17.05.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. DocMi.15.07

doi: 10.3205/17dgnc468, urn:nbn:de:0183-17dgnc4681

Published: June 9, 2017

© 2017 Reinke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Objective: Virus are known as origin for various diseases. Vaccination against human papilloma virus is established for avoiding cervix carcinoma. Furthermore, Parvovirus B19 seems to play a role in appearance of cardiomyopathy and rheumatoid arthritis. Disc herniation is generally considered as a degenerative disease. But can we explain a disc herniation in youth and young adults to be degenerative? For evaluation the pathogenicity of parvovirus B19 related to the onset of disc herniation the present study was designed.

Methods: Out of 47 patients with lumbar or cervical disc herniation that underwent spinal surgery, the disc herniation was tested for Parvovirus by PCR (cervical prolapse n=16; lumbar prolapse n=31). After surgical excision of the herniated disc the sample tissue was placed in RNA-later and stored at -20°C until the virological analysis was made. Initially samples of the patients’ blood serum were screened with ELISA for IgG antibodies. After the mechanical comminution of the disc material the nucleic acid was automatically extracted. Subsequently the viral nucleic acid was detected with quantitative polymerase chain reaction. Prion Protein DNA was added to the lysis buffer before the extraction as a combined extraction and inhibitioncontrol. The validity of the PCR tests was assured through positive controls and standards, which need to be in a certain target range

Results: In 21 samples we could detect positive PCR-results for Parvovirus, while the internal control is in the expected normal range. The serological testing of our patient collective showed a 76.6 % IgG-positive result (n=36), corresponding to the available data in literature for the spread of infection in the average population. Consequently 58.3% of serological positive patients and 44.9% of all patients showed an infection with Parvovirus B19 in herniated disc.

Conclusion: The results of Parvovirus in herniated disc are surprising. This evidence suggests a possible correlation between the Parvovirus and the appearance of a disc herniation.