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86th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

13.05. - 16.05.2015, Berlin

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Genetic Polymorphism of NOS2 -954G/C in Nasal Polyposis. A case Control Study in a Population Group of Northern Romania

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  • corresponding author Iuliu Vlad Catana - Cmi Dr. Catana ORL, Cluj-Napoca, Romania
  • Andreea Catana - Iuliu Hatieganu University of Medicine, Human Genetics, Cluj-Napoca, Romania
  • Alma Maniu - Iuliu Hatieganu University of Medicine, Human Genetics, Cluj-Napoca, Romania
  • Marcel Cosgarea - Iuliu Hatieganu University of Medicine, ENT clinic, Cluj-Napoca, Romania

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 86. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Berlin, 13.-16.05.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. Doc15hnod575

doi: 10.3205/15hnod575, urn:nbn:de:0183-15hnod5755

Published: March 26, 2015

© 2015 Catana et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License. You are free: to Share - to copy, distribute and transmit the work, provided the original author and source are credited. See license information at http://creativecommons.org/licenses/by-nc-nd/3.0/.

Outline

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Background: Polymorphisms for genes encoding chemosensitive signalling proteins like NOS2, might contribute to the variability in individual susceptibility to nasal polyposis. NO produced by the inducible NO synthase enzyme NOS2A is generated at high levels in certain types of inflammation, so that the role of NOS2 might be important in nasal polyposis etiopathogeny.

Study Design: This is a cross-sectional, randomized, case control study for the evaluation of the frequency of -954G/C NOS2 polymorphism alleles among patients with nasal polyposis.

Subjects: The study included 92 cases of nasal polyposis diagnosed patients (nasal endoscopy and CT scan examination), and 107 healthy unrelated controls.

Methods: -954G/C NOS2 genotyping was carried out using PCR amplification of relevant gene fragment was followed by restriction enzyme digestion. Detection of the variant alleles was determined through analysis of resulting restriction fragment length polymorphism (RFLP) followed by gel electrophoresis.

Results: Molecular analysis revealed an increased frequency of -954G/CNOS2 variant allele in the study group compared to the control group (p=0.043; OR= 1.77; CI=1.02-3.09).

Conclusions: The main finding of our study is that mutant genotype of -954G/C NOS2 is considered to be a risk factor for nasal polyposis development.

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