The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_F777C7E061E2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning.
Journal
Plant Physiology
Author(s)
de Carbonnel M., Davis P., Roelfsema M.R., Inoue S., Schepens I., Lariguet P., Geisler M., Shimazaki K., Hangarter R., Fankhauser C.
ISSN
1532-2548[electronic], 0032-0889[linking]
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
152
Number
3
Pages
1391-1405
Language
english
Abstract
In Arabidopsis (Arabidopsis thaliana), the blue light photoreceptor phototropins (phot1 and phot2) fine-tune the photosynthetic status of the plant by controlling several important adaptive processes in response to environmental light variations. These processes include stem and petiole phototropism (leaf positioning), leaf flattening, stomatal opening, and chloroplast movements. The PHYTOCHROME KINASE SUBSTRATE (PKS) protein family comprises four members in Arabidopsis (PKS1-PKS4). PKS1 is a novel phot1 signaling element during phototropism, as it interacts with phot1 and the important signaling element NONPHOTOTROPIC HYPOCOTYL3 (NPH3) and is required for normal phot1-mediated phototropism. In this study, we have analyzed more globally the role of three PKS members (PKS1, PKS2, and PKS4). Systematic analysis of mutants reveals that PKS2 (and to a lesser extent PKS1) act in the same subset of phototropin-controlled responses as NPH3, namely leaf flattening and positioning. PKS1, PKS2, and NPH3 coimmunoprecipitate with both phot1-green fluorescent protein and phot2-green fluorescent protein in leaf extracts. Genetic experiments position PKS2 within phot1 and phot2 pathways controlling leaf positioning and leaf flattening, respectively. NPH3 can act in both phot1 and phot2 pathways, and synergistic interactions observed between pks2 and nph3 mutants suggest complementary roles of PKS2 and NPH3 during phototropin signaling. Finally, several observations further suggest that PKS2 may regulate leaf flattening and positioning by controlling auxin homeostasis. Together with previous findings, our results indicate that the PKS proteins represent an important family of phototropin signaling proteins.
Keywords
Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Cell Size, Chloroplasts/physiology, Homeostasis, Indoleacetic Acids/metabolism, Light, Mutation, Phototropins/metabolism, Plant Leaves/physiology, Plant Stomata/physiology
Pubmed
Web of science
Open Access
Yes
Create date
26/01/2010 12:29
Last modification date
20/08/2019 16:23
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