Ectodysplasin/NF-κB Promotes Mammary Cell Fate via Wnt/β-catenin Pathway.

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Serval ID
serval:BIB_F33C6DEF90DA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ectodysplasin/NF-κB Promotes Mammary Cell Fate via Wnt/β-catenin Pathway.
Journal
Plos Genetics
Author(s)
Voutilainen M., Lindfors P.H., Trela E., Lönnblad D., Shirokova V., Elo T., Rysti E., Schmidt-Ullrich R., Schneider P., Mikkola M.L.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
11
Number
11
Pages
e1005676
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Abstract
Mammary gland development commences during embryogenesis with the establishment of a species typical number of mammary primordia on each flank of the embryo. It is thought that mammary cell fate can only be induced along the mammary line, a narrow region of the ventro-lateral skin running from the axilla to the groin. Ectodysplasin (Eda) is a tumor necrosis factor family ligand that regulates morphogenesis of several ectodermal appendages. We have previously shown that transgenic overexpression of Eda (K14-Eda mice) induces formation of supernumerary mammary placodes along the mammary line. Here, we investigate in more detail the role of Eda and its downstream mediator transcription factor NF-κB in mammary cell fate specification. We report that K14-Eda mice harbor accessory mammary glands also in the neck region indicating wider epidermal cell plasticity that previously appreciated. We show that even though NF-κB is not required for formation of endogenous mammary placodes, it is indispensable for the ability of Eda to induce supernumerary placodes. A genome-wide profiling of Eda-induced genes in mammary buds identified several Wnt pathway components as potential transcriptional targets of Eda. Using an ex vivo culture system, we show that suppression of canonical Wnt signalling leads to a dose-dependent inhibition of supernumerary placodes in K14-Eda tissue explants.
Keywords
Animals, Cell Differentiation/genetics, Ectodysplasins/biosynthesis, Ectodysplasins/genetics, Embryo, Mammalian, Gene Expression Regulation, Developmental, Hair Follicle/growth & development, Humans, Mammary Glands, Human/cytology, Mammary Glands, Human/growth & development, Mice, Morphogenesis/genetics, NF-kappa B/genetics, NF-kappa B/metabolism, Wnt Signaling Pathway/genetics
Pubmed
Web of science
Open Access
Yes
Create date
04/01/2016 9:52
Last modification date
20/08/2019 16:20
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