Choroidal mast cells in retinal pathology: a potential target for intervention.

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_D4CF9AE4C269
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Choroidal mast cells in retinal pathology: a potential target for intervention.
Journal
American Journal of Pathology
Author(s)
Bousquet E., Zhao M., Thillaye-Goldenberg B., Lorena V., Castaneda B., Naud M.C., Bergin C., Besson-Lescure B., Behar-Cohen F., de Kozak Y.
ISSN
1525-2191 (Electronic)
ISSN-L
0002-9440
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
185
Number
8
Pages
2083-2095
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Mast cells are important in the initiation of ocular inflammation, but the consequences of mast cell degranulation on ocular pathology remain uncharacterized. We induced mast cell degranulation by local subconjunctival injection of compound 48/80. Initial degranulation of mast cells was observed in the choroid 15 minutes after the injection and increased up to 3 hours after injection. Clinical signs of anterior segment inflammation paralleled mast cell degranulation. With the use of optical coherence tomography, dilation of choroidal vessels and serous retinal detachments (SRDs) were observed and confirmed by histology. Subconjunctival injection of disodium cromoglycate significantly reduced the rate of SRDs, demonstrating the involvement of mast cell degranulation in posterior segment disorders. The infiltration of polymorphonuclear and macrophage cells was associated with increased ocular media concentrations of tumor necrosis factor-α, CXCL1, IL-6, IL-5, chemokine ligand 2, and IL-1β. Analysis of the amounts of vascular endothelial growth factor and IL-18 showed an opposite evolution of vascular endothelial growth factor compared with IL-18 concentrations, suggesting that they regulate each other's production. These findings suggest that the local degranulation of ocular mast cells provoked acute ocular inflammation, dilation, increased vascular permeability of choroidal vessels, and SRDs. The involvement of mast cells in retinal diseases should be further investigated. The pharmacologic inhibition of mast cell degranulation may be a potential target for intervention.
Keywords
Animals, Capillary Permeability/drug effects, Cell Degranulation/drug effects, Chemokines/metabolism, Choroid/drug effects, Choroid/metabolism, Cytokines/metabolism, Female, Mast Cells/drug effects, Mast Cells/metabolism, Rats, Rats, Inbred Lew, Retina/drug effects, Retina/metabolism, Tomography, Optical Coherence, p-Methoxy-N-methylphenethylamine/pharmacology
Pubmed
Web of science
Create date
04/08/2015 7:26
Last modification date
20/08/2019 15:54
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