Automated computer-assisted quantitative analysis of intact murine lungs at the alveolar scale.

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Version: Final published version
Serval ID
serval:BIB_D451D9FC6EE2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Automated computer-assisted quantitative analysis of intact murine lungs at the alveolar scale.
Journal
PloS one
Author(s)
Lovric G., Vogiatzis Oikonomidis I., Mokso R., Stampanoni M., Roth-Kleiner M., Schittny J.C.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
12
Number
9
Pages
e0183979
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Using state-of-the-art X-ray tomographic microscopy we can image lung tissue in three dimensions in intact animals down to a micrometer precision. The structural complexity and hierarchical branching scheme of the lung at this level of details, however, renders the extraction of biologically relevant quantities particularly challenging. We have developed a methodology for a detailed description of lung inflation patterns by measuring the size and the local curvature of the parenchymal airspaces. These quantitative tools for morphological and topological analyses were applied to high-resolution murine 3D lung image data, inflated at different pressure levels under immediate post mortem conditions. We show for the first time direct indications of heterogeneous intra-lobar and inter-lobar distension patterns at the alveolar level. Furthermore, we did not find any indication that a cyclic opening-and-collapse (recruitment) of a large number of alveoli takes place.

Keywords
Animals, Automation, Imaging, Three-Dimensional/methods, Mice, Microscopy, Pulmonary Alveoli/diagnostic imaging, Tomography, X-Ray Computed
Pubmed
Web of science
Open Access
Yes
Create date
09/10/2017 9:28
Last modification date
20/08/2019 15:54
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