The inhibition of fat cell proliferation by n-3 fatty acids in dietary obese mice.

Details

Ressource 1Download: BIB_C2A61BCE5407.P001.pdf (318.71 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_C2A61BCE5407
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The inhibition of fat cell proliferation by n-3 fatty acids in dietary obese mice.
Journal
Lipids in Health and Disease
Author(s)
Hensler M., Bardova K., Jilkova Z.M., Wahli W., Meztger D., Chambon P., Kopecky J., Flachs P.
ISSN
1476-511X (Electronic)
ISSN-L
1476-511X
Publication state
Published
Issued date
2011
Volume
10
Number
1
Pages
128
Language
english
Abstract
ABSTRACT: BACKGROUND: Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) of marine origin exert multiple beneficial effects on health. Our previous study in mice showed that reduction of adiposity by LC n-3 PUFA was associated with both, a shift in adipose tissue metabolism and a decrease in tissue cellularity. The aim of this study was to further characterize the effects of LC n-3 PUFA on fat cell proliferation and differentiation in obese mice. METHODS: A model of inducible and reversible lipoatrophy (aP2-Cre-ERT2 PPARgammaL2/L2 mice) was used, in which the death of mature adipocytes could be achieved by a selective ablation of peroxisome proliferator-activated receptor gamma in response to i.p. injection of tamoxifen. Before the injection, obesity was induced in male mice by 8-week-feeding a corn oil-based high-fat diet (cHF) and, subsequently, mice were randomly assigned (day 0) to one of the following groups: (i) mice injected by corn-oil-vehicle only, i.e."control" mice, and fed cHF; (ii) mice injected by tamoxifen in corn oil, i.e. "mutant" mice, fed cHF; (iii) control mice fed cHF diet with 15% of dietary lipids replaced by LC n-3 PUFA concentrate (cHF+F); and (iv) mutant mice fed cHF+F. Blood and tissue samples were collected at days 14 and 42. RESULTS: Mutant mice achieved a maximum weight loss within 10 days post-injection, followed by a compensatory body weight gain, which was significantly faster in the cHF as compared with the cHF+F mutant mice. Also in control mice, body weight gain was depressed in response to dietary LC n-3 PUFA. At day 42, body weights in all groups stabilized, with no significant differences in adipocyte size between the groups, although body weight and adiposity was lower in the cHF+F as compared with the cHF mice, with a stronger effect in the mutant than in control mice. Gene expression analysis documented depression of adipocyte maturation during the reconstitution of adipose tissue in the cHF+F mutant mice. CONCLUSION: Dietary LC n-3 PUFA could reduce both hypertrophy and hyperplasia of fat cells in vivo. Results are in agreement with the involvement of fat cell turnover in control of adiposity.
Keywords
Adipocytes/drug effects, Adipocytes/pathology, Animals, Cell Proliferation/drug effects, Corn Oil/adverse effects, Drug Evaluation, Preclinical, Epididymis/metabolism, Epididymis/pathology, Fatty Acids, Omega-3/pharmacology, Gene Expression, Gene Knockout Techniques, Male, Mice, Mice, Transgenic, Obesity/chemically induced, Obesity/prevention & control, PPAR alpha/genetics, PPAR alpha/metabolism, PPAR gamma/genetics, Prostaglandin-Endoperoxide Synthases/genetics, Prostaglandin-Endoperoxide Synthases/metabolism, Proteins/genetics, Proteins/metabolism, Stearoyl-CoA Desaturase/genetics, Stearoyl-CoA Desaturase/metabolism, Trans-Activators/genetics, Trans-Activators/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
15/08/2011 9:11
Last modification date
20/08/2019 15:37
Usage data