Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5
Details
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State: Public
Version: Final published version
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
State: Public
Version: Final published version
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_B8D897551E74
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5
Journal
Journal of Infectious Diseases
ISSN
0022-1899
Publication state
Published
Issued date
04/1991
Peer-reviewed
Oui
Volume
163
Number
4
Pages
769-72
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
To investigate whether immunization with Escherichia coli J5 boiled cells induces antibodies directed at deep core structures, antibodies against J5 lipopolysaccharide (LPS), Re LPS, and lipid A were measured in the serum of 70 volunteers before and 2 weeks after immunization. To improve the sensitivity and the specificity of ELISA, complexes of core LPS with high-density lipoproteins were used instead of free core LPS as antigens. A median three-fold increase in antibodies directed against J5 LPS was observed, but no significant increase in the antibodies against Re LPS or lipid A was found. Since J5 antiserum did not react with several smooth LPS or with Re LPS and lipid A, cross-reactivity could not be demonstrated. Thus, immunization of volunteers with E. coli J5 produced a modest specific antibody response against J5 LPS. The mechanism of protection previously observed with J5 antiserum remains unclear.
Keywords
Antibodies, Bacterial/*biosynthesis/blood
Bacterial Vaccines/*immunology
Enzyme-Linked Immunosorbent Assay
Escherichia coli/*immunology
Humans
Immune Sera/immunology
*Immunization
Immunoglobulin G/biosynthesis
Immunoglobulin M/biosynthesis
Lipopolysaccharides/*immunology
Lipoproteins, HDL/immunology
Pubmed
Web of science
Open Access
Yes
Create date
19/01/2008 19:12
Last modification date
14/02/2022 8:56