Identification of <i>Salvia haenkei</i> as gerosuppressant agent by using an integrated senescence-screening assay.

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Version: Final published version
Serval ID
serval:BIB_B3FA03C03F0D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of <i>Salvia haenkei</i> as gerosuppressant agent by using an integrated senescence-screening assay.
Journal
Aging
Author(s)
Matic I., Revandkar A., Chen J., Bisio A., Dall'Acqua S., Cocetta V., Brun P., Mancino G., Milanese M., Mattei M., Montopoli M., Alimonti A.
ISSN
1945-4589 (Electronic)
ISSN-L
1945-4589
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
8
Number
12
Pages
3223-3240
Language
english
Abstract
Cellular senescence is a stable cell cycle arrest that is the causative process of aging. The PI3K/AKT/mTOR pathway is implicated in the control of cellular senescence and inhibitors of this pathway have been successfully used for life span prolongation experiments in mammals. PTEN is the major regulator of the PI3K/AKT/mTOR pathway and loss of PTEN promotes a senescence response termed PICS. Here we report a novel-screening assay, for the identification of compounds that block different types of senescence response. By testing a library of more than 3000 natural and chemical compounds in PTEN deficient cells we have found that an extract from <i>Salvia haenkei</i> (SH), a native plant of Bolivia is a potent inhibitor of PICS. SH also decreases replicative and UV-mediated senescence in human primary fibroblasts and in a model of <i>in vitro</i> reconstructed human epidermis. Mechanistically, SH treatment affects senescence driven by UV by interfering with IL1-α signalling. Pre-clinical and clinical testing of this extract by performing toxicity and irritability evaluation <i>in vitro</i> also demonstrate the safety of SH extract for clinical use as anti-aging skin treatment.
Keywords
Animals, Cell Proliferation/drug effects, Cell Proliferation/radiation effects, Cells, Cultured, Cellular Senescence/drug effects, Cellular Senescence/radiation effects, Fibroblasts/drug effects, Gene Expression Regulation, Humans, Hydrogen Peroxide/metabolism, Interleukin-1alpha/genetics, Interleukin-1alpha/metabolism, Mice, Oxidative Stress, Plant Extracts/chemistry, Plant Extracts/pharmacology, Salvia/chemistry, PICS, Salvia haenkei, gerosuppressant, senescence, senescence-screening assay
Pubmed
Web of science
Create date
18/02/2018 23:04
Last modification date
20/08/2019 15:22
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