Cellular Barcoding Identifies Clonal Substitution as a Hallmark of Local Recurrence in a Surgical Model of Head and Neck Squamous Cell Carcinoma.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_B1CDCE626F72
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cellular Barcoding Identifies Clonal Substitution as a Hallmark of Local Recurrence in a Surgical Model of Head and Neck Squamous Cell Carcinoma.
Journal
Cell reports
Author(s)
Roh V., Abramowski P., Hiou-Feige A., Cornils K., Rivals J.P., Zougman A., Aranyossy T., Thielecke L., Truan Z., Mermod M., Monnier Y., Prassolov V., Glauche I., Nowrouzi A., Abdollahi A., Fehse B., Simon C., Tolstonog G.V.
ISSN
2211-1247 (Electronic)
Publication state
Published
Issued date
20/11/2018
Peer-reviewed
Oui
Volume
25
Number
8
Pages
2208-2222.e7
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Local recurrence after surgery for head and neck squamous cell carcinoma (HNSCC) remains a common event associated with a dismal prognosis. Improving this outcome requires a better understanding of cancer cell populations that expand from postsurgical minimal residual disease (MRD). Therefore, we assessed clonal dynamics in a surgical model of barcoded HNSCC growing in the submental region of immunodeficient mice. Clonal substitution and massive reduction of clonal heterogeneity emerged as hallmarks of local recurrence, as the clones dominating in less heterogeneous recurrences were scarce in their matched primary tumors. These lineages were selected by their ability to persist after surgery and competitively expand from MRD. Clones enriched in recurrences exhibited both private and shared genetic features and likely originated from ancestors shared with clones dominating in primary tumors. They demonstrated high invasiveness and epithelial-to-mesenchymal transition, eventually providing an attractive target for obtaining better local control for these tumors.
Keywords
DNA barcoding, LeGO vectors, RGB marking, clonal heterogeneity, clonal substitution, epithelial-mesenchymal transition, head and neck squamous cell carcinoma, invasion, recurrent tumor-initiating clones
Pubmed
Web of science
Open Access
Yes
Create date
10/12/2018 18:14
Last modification date
20/08/2019 15:20
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