A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_ACF3DD1B42CD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.
Journal
Journal of Medical Microbiology
Author(s)
Prod'hom G., Durussel C., Greub G.
ISSN
1473-5644 (Electronic)
ISSN-L
0022-2615
Publication state
Published
Issued date
2013
Volume
62
Number
Pt 5
Pages
773-777
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3% for Enterobacteriaceae, and 0.7 and 0.1% for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.
Keywords
Anti-Bacterial Agents/pharmacology, Bacteriological Techniques, Drug Resistance, Bacterial, Gram-Negative Bacteria/classification, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/classification, Gram-Positive Bacteria/drug effects, Humans, Microbial Sensitivity Tests, Sensitivity and Specificity
Pubmed
Web of science
Open Access
Yes
Create date
04/07/2013 19:50
Last modification date
20/08/2019 15:16
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