Identification of Genes Affecting Vacuole Membrane Fragmentation in Saccharomyces cerevisiae.

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serval:BIB_A959689C4C18
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of Genes Affecting Vacuole Membrane Fragmentation in Saccharomyces cerevisiae.
Journal
PLoS One
Author(s)
Michaillat L., Mayer A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2013
Volume
8
Number
2
Pages
e54160
Language
english
Abstract
The equilibrium of membrane fusion and fission influences the volume and copy number of organelles. Fusion of yeast vacuoles has been well characterized but their fission and the mechanisms determining vacuole size and abundance remain poorly understood. We therefore attempted to systematically characterize factors necessary for vacuole fission. Here, we present results of an in vivo screening for deficiencies in vacuolar fragmentation activity of an ordered collection deletion mutants, representing 4881 non-essential genes of the yeast Saccharomyces cerevisiae. The screen identified 133 mutants with strong defects in vacuole fragmentation. These comprise numerous known fragmentation factors, such as the Fab1p complex, Tor1p, Sit4p and the V-ATPase, thus validating the approach. The screen identified many novel factors promoting vacuole fragmentation. Among those are 22 open reading frames of unknown function and three conspicuous clusters of proteins with known function. The clusters concern the ESCRT machinery, adaptins, and lipases, which influence the production of diacylglycerol and phosphatidic acid. A common feature of these factors of known function is their capacity to change membrane curvature, suggesting that they might promote vacuole fragmentation via this property.
Pubmed
Web of science
Open Access
Yes
Create date
07/03/2013 8:02
Last modification date
20/08/2019 15:13
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