Mouse mammary tumour virus related sequences are present in human DNA

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Serval ID
serval:BIB_A5E821E01C94
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mouse mammary tumour virus related sequences are present in human DNA
Journal
Nucleic Acids Research
Author(s)
May F. E., Westley B. R., Rochefort H., Buetti E., Diggelmann H.
ISSN
0305-1048 (Print)
Publication state
Published
Issued date
06/1983
Volume
11
Number
12
Pages
4127-39
Notes
Comparative Study Journal Article Research Support, Non-U.S. Gov't --- Old month value: Jun 25
Abstract
MuMTV-related sequences have been identified in the DNA of human breast cancer cells using the Southern transfer technique and hybridisation with cloned MuMTV DNA under conditions in which partially mismatched sequences form stable hybrids. Hybridisation with cloned fragments of the MuMTV genome showed that the gag-pol region shares the most homology (estimated to be greater than 80%) with the human MuMTV-related sequences, however, DNA fragments partially homologous to the MuMTV LTR, gag ad env regions were also detected. Analysis of several human DNA samples suggests that the majority of the human MuMTV-related sequences are genetically transmitted but additional Eco R1 fragments were detected in the DNA of one out of three breast cancer cell lines, MCF7. These sequences are potential probes for the human MuMTV-related retroviral sequences and will allow their possible role in human breast cancer to be evaluated.
Keywords
Animals Base Sequence Breast Neoplasms/genetics DNA/*analysis DNA, Viral/*analysis Humans Mammary Tumor Virus, Mouse/*genetics Mice Nucleic Acid Hybridization
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Web of science
Create date
25/01/2008 14:25
Last modification date
20/08/2019 15:11
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