Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response.

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Version: Final published version
Serval ID
serval:BIB_A52A9EB92208
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response.
Journal
Investigative Ophthalmology and Visual Science
Author(s)
Kostic C., Crippa S.V., Martin C., Kardon R.H., Biel M., Arsenijevic Y., Kawasaki A.
ISSN
1552-5783 (Electronic)
ISSN-L
0146-0404
Publication state
Published
Issued date
05/2016
Peer-reviewed
Oui
Volume
57
Number
6
Pages
2501-2508
Language
english
Abstract
PURPOSE: This study aims to identify which aspects of the pupil light reflex are most influenced by rods and cones independently by analyzing pupil recordings from different mouse models of photoreceptor deficiency.
METHODS: One-month-old wild type (WT), rodless (Rho-/-), coneless (Cnga3-/-), or photoreceptor less (Cnga3-/-; Rho-/- or Gnat1-/-) mice were subjected to brief red and blue light stimuli of increasing intensity. To describe the initial dynamic response to light, the maximal pupillary constriction amplitudes and the derivative curve of the first 3 seconds were determined. To estimate the postillumination phase, the constriction amplitude at 9.5 seconds after light termination was related to the maximal constriction amplitude.
RESULTS: Rho-/- mice showed decreased constriction amplitude but more prolonged pupilloconstriction to all blue and red light stimuli compared to wild type mice. Cnga3-/- mice had constriction amplitudes similar to WT however following maximal constriction, the early and rapid dilation to low intensity blue light was decreased. To high intensity blue light, the Cnga3-/- mice demonstrated marked prolongation of the pupillary constriction. Cnga3-/-; Rho-/- mice had no pupil response to red light of low and medium intensity.
CONCLUSIONS: From specific gene defective mouse models which selectively voided the rod or cone function, we determined that mouse rod photoreceptors are highly contributing to the pupil response to blue light stimuli but also to low and medium red stimuli. We also observed that cone cells mainly drive the partial rapid dilation of the initial response to low blue light stimuli. Thus photoreceptor dysfunction can be derived from chromatic pupillometry in mouse models.
Pubmed
Web of science
Open Access
Yes
Create date
12/05/2016 8:04
Last modification date
20/08/2019 15:10
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