Cellular viral rebound after cessation of potent antiretroviral therapy predicted by levels of multiply spliced HIV-1 RNA encoding nef

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Serval ID
serval:BIB_A06B4AC25E63
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cellular viral rebound after cessation of potent antiretroviral therapy predicted by levels of multiply spliced HIV-1 RNA encoding nef
Journal
Journal of Infectious Diseases
Author(s)
Fischer M., Joos B., Hirschel B., Bleiber G., Weber R., Günthard H.F.
Working group(s)
Swiss HIV Cohort Study
ISSN
0022-1899 (Print)
ISSN-L
0022-1899
Publication state
Published
Issued date
2004
Volume
190
Number
11
Pages
1979-1988
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
To characterize newly arising replication of human immunodeficiency virus (HIV) type 1 in vivo at the cellular level, distinct viral RNA species in peripheral blood mononuclear cells (PBMCs) from HIV-1-infected patients were monitored during 2 weeks of structured treatment interruption (STI). HIV-1 RNA encoding tat/rev and PBMC-associated virions were almost completely depleted during antiretroviral therapy and emerged simultaneously after 2 weeks of STI, thus specifically reflecting productive viral infection at the cellular level. The magnitude of these correlates of reappearing cellular viral replication was predicted by during-therapy levels of nef transcripts in PBMCs. Significant rebound of plasma viremia, representing the progeny of a broader range of anatomical compartments, preceded and predicted productive infection in PBMCs. Thus, cellular viral rebound in PBMCs likely was primed before STI by the expression of nef in HIV-1-infected PBMCs that lacked virion production and was subsequently triggered by the plasma viremia that preceded the recurrence of productively infected PBMCs.
Keywords
Anti-HIV Agents/therapeutic use, Gene Products, nef/analysis, Gene Products, nef/biosynthesis, Gene Products, rev/metabolism, Gene Products, tat/metabolism, HIV Infections/blood, HIV Infections/drug therapy, HIV Infections/virology, HIV-1/genetics, HIV-1/isolation & purification, Leukocytes, Mononuclear/virology, Neutrophils/virology, RNA, Viral/analysis, RNA, Viral/biosynthesis
Pubmed
Web of science
Open Access
Yes
Create date
13/07/2018 9:00
Last modification date
14/02/2022 7:56
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