Differential expression and hypoxic regulation of adenosine receptors and TRPC channels in the developing heart.
Details
State: Public
Version: After imprimatur
Serval ID
serval:BIB_9910DD11D755
Type
A Master's thesis.
Publication sub-type
Master (thesis) (master)
Collection
Publications
Institution
Title
Differential expression and hypoxic regulation of adenosine receptors and TRPC channels in the developing heart.
Director(s)
Raddatz E.
Codirector(s)
Robin E.
Institution details
Université de Lausanne, Faculté de biologie et médecine
Publication state
Accepted
Issued date
2012
Language
english
Number of pages
33
Abstract
Objectives: To characterize the modifications of gene expression of adenosine receptors (AR), TRPC channels, HIF-1α and iNOS during the early cardiogenesis in response to chronic hypoxia exposure. Methods: 4-day-old chick embryos were subjected in ovo to 6H, 12H and 24H of hypoxia (10% O2). The mRNA expression was quantified by RT-qPCR. Results: The targeted genes were found to be expressed at mRNA level with a differential expression pattern within the heart. Hypoxia has no significant effect on mRNA expression of ARs, TRPCs channels and iNOS within the heart. By contrast, HIF-1α mRNA expression shows a tendency to be down-regulated by hypoxia. Conclusion: These results suggest that an intrauterine oxygen lack does not significantly affect expression of genes involved in adenosine signaling and in calcium handling by store operated channels (TRPC).
Keywords
Adenosine receptors, TRPC, HIF-1α, iNOS, embryo, heart, hypoxia
Create date
10/09/2013 9:37
Last modification date
20/08/2019 16:00
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