Sorting live stem cells based on sox2 mRNA expression.

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State: Public
Version: author
Serval ID
serval:BIB_961F08073442
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sorting live stem cells based on sox2 mRNA expression.
Journal
Plos One
Author(s)
Larsson H.M., Lee S.T., Roccio M., Velluto D., Lutolf M.P., Frey P., Hubbell J.A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
7
Number
11
Pages
e49874
Language
english
Notes
Publication types: Journal Article
Abstract
While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based on the presence of any expressed mRNA and in principle could be extremely useful to sort rare cell populations from primary isolates. We show here how stem cells can be purified from mixed cell populations by sorting based on MBs. Specifically, we designed molecular beacons targeting Sox2, a well-known stem cell marker for murine embryonic (mES) and neural stem cells (NSC). One of our designed molecular beacons displayed an increase in fluorescence compared to a nonspecific molecular beacon both in vitro and in vivo when tested in mES and NSCs. We sorted Sox2-MB(+)SSEA1(+) cells from a mixed population of 4-day retinoic acid-treated mES cells and effectively isolated live undifferentiated stem cells. Additionally, Sox2-MB(+) cells isolated from primary mouse brains were sorted and generated neurospheres with higher efficiency than Sox2-MB(-) cells. These results demonstrate the utility of MBs for stem cell sorting in an mRNA-specific manner.
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2013 18:21
Last modification date
20/08/2019 14:58
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