The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7.

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License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_941E4C88E68D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7.
Journal
Cell reports
Author(s)
Trofimenko E., Homma Y., Fukuda M., Widmann C.
ISSN
2211-1247 (Electronic)
Publication state
Published
Issued date
02/11/2021
Peer-reviewed
Oui
Volume
37
Number
5
Pages
109945
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Endocytosis and endosome dynamics are controlled by proteins of the small GTPase Rab family. Besides possible recycling routes to the plasma membrane and various organelles, previously described endocytic pathways (e.g., clathrin-mediated endocytosis, macropinocytosis, CLIC/GEEC pathway) all appear to funnel the endocytosed material to Rab5-positive early endosomes that then mature into Rab7-positive late endosomes/lysosomes. By studying the uptake of a series of cell-penetrating peptides (CPPs), we identify an endocytic pathway that moves material to nonacidic Lamp1-positive late endosomes. Trafficking via this endocytic route is fully independent of Rab5 and Rab7 but requires the Rab14 protein. The pathway taken by CPPs differs from the conventional Rab5-dependent endocytosis at the stage of vesicle formation already, as it is not affected by a series of compounds that inhibit macropinocytosis or clathrin-mediated endocytosis. The Rab14-dependent pathway is also used by physiological cationic molecules such as polyamines and homeodomains found in homeoproteins.
Keywords
Lamp1, Rab14, Rab5, Rab7, cell-penetrating peptides, endocytosis, endosomes, homeodomains, homeoproteins, polyamines
Pubmed
Open Access
Yes
Create date
08/11/2021 15:44
Last modification date
12/11/2021 7:11
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