Spinal Cord T-Cell Infiltration in the Rat Spared Nerve Injury Model: A Time Course Study.

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Serval ID
serval:BIB_929F9D610E0B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spinal Cord T-Cell Infiltration in the Rat Spared Nerve Injury Model: A Time Course Study.
Journal
International journal of molecular sciences
Author(s)
Gattlen C., Clarke C.B., Piller N., Kirschmann G., Pertin M., Decosterd I., Gosselin R.D., Suter M.R.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
09/03/2016
Peer-reviewed
Oui
Volume
17
Number
3
Pages
352
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The immune system is involved in the development of neuropathic pain. In particular, the infiltration of T-lymphocytes into the spinal cord following peripheral nerve injury has been described as a contributor to sensory hypersensitivity. We used the spared nerve injury (SNI) model of neuropathic pain in Sprague Dawley adult male rats to assess proliferation, and/or protein/gene expression levels for microglia (Iba1), T-lymphocytes (CD2) and cytotoxic T-lymphocytes (CD8). In the dorsal horn ipsilateral to SNI, Iba1 and BrdU stainings revealed microglial reactivity and proliferation, respectively, with different durations. Iba1 expression peaked at D4 and D7 at the mRNA and protein level, respectively, and was long-lasting. Proliferation occurred almost exclusively in Iba1 positive cells and peaked at D2. Gene expression observation by RT-qPCR array suggested that T-lymphocytes attracting chemokines were upregulated after SNI in rat spinal cord but only a few CD2/CD8 positive cells were found. A pronounced infiltration of CD2/CD8 positive T-cells was seen in the spinal cord injury (SCI) model used as a positive control for lymphocyte infiltration. Under these experimental conditions, we show early and long-lasting microglia reactivity in the spinal cord after SNI, but no lymphocyte infiltration was found.

Keywords
Animals, Antigens, CD2/genetics, Antigens, CD8/genetics, Calcium-Binding Proteins/genetics, Cell Proliferation, Chemokines/immunology, Disease Models, Animal, Gene Expression, Male, Microfilament Proteins/genetics, Microglia/metabolism, Microglia/pathology, Microglia/physiology, Neuralgia, Peripheral Nerve Injuries/complications, Peripheral Nerve Injuries/immunology, Rats, Rats, Sprague-Dawley, Spinal Cord Injuries/etiology, Spinal Cord Injuries/immunology, Spinal Cord Injuries/physiopathology, T-Lymphocytes/metabolism, T-Lymphocytes/pathology, T-Lymphocytes/physiology
Pubmed
Open Access
Yes
Create date
29/03/2016 11:52
Last modification date
20/08/2019 14:55
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