Toxic effects of brake wear particles on epithelial lung cells in vitro

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Version: Final published version
Serval ID
serval:BIB_834D29655A82
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Toxic effects of brake wear particles on epithelial lung cells in vitro
Journal
Particle and Fibre Toxicology
Author(s)
Gasser Michael, Riediker Michael, Mueller Loretta, Perrenoud Alain, Blank Fabian, Gehr Peter, Rothen-Rutishauser Barbara
ISSN
1743-8977 (Electronic)
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
6
Number
30
Pages
[13]
Language
english
Notes
SAPHIRID:81301
Abstract
Background: Fine particulate matter originating from traffic correlates with increased morbidity and mortality. An important source of traffic particles is brake wear of cars which contributes up to 20% of the total traffic emissions. The aim of this study was to evaluate potential toxicological effects of human epithelial lung cells exposed to freshly generated brake wear particles. Results: An exposure box was mounted around a car's braking system. Lung cells cultured at the air-liquid interface were then exposed to particles emitted from two typical braking behaviours ("full stop" and "normal deceleration"). The particle size distribution as well as the brake emission components like metals and carbons was measured on-line, and the particles deposited on grids for transmission electron microscopy were counted. The tight junction arrangement was observed by laser scanning microscopy. Cellular responses were assessed by measurement of lactate dehydrogenase (cytotoxicity), by investigating the production of reactive oxidative species and the release of the pro-inflammatory mediator interleukin-8. The tight junction protein occludin density decreased significantly (p < 0.05) with increasing concentrations of metals on the particles (iron, copper and manganese, which were all strongly correlated with each other). Occludin was also negatively correlated with the intensity of reactive oxidative species. The concentrations of interleukin-8 were significantly correlated with increasing organic carbon concentrations. No correlation was observed between occludin and interleukin-8, nor between reactive oxidative species and interleukin-8. Conclusion: These findings suggest that the metals on brake wear particles damage tight junctions with a mechanism involving oxidative stress. Brake wear particles also increase pro-inflammatory responses. However, this might be due to another mechanism than via oxidative stress. [Authors]
Keywords
Vehicle Emissions , Particulate Matter , Particle Size , Epithelial Cells , Toxicity Tests
Pubmed
Web of science
Open Access
Yes
Create date
19/01/2010 17:30
Last modification date
20/08/2019 14:43
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