Propofol anesthesia impairs the maturation and survival of adult-born hippocampal neurons.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_829F3A1BA6F6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Propofol anesthesia impairs the maturation and survival of adult-born hippocampal neurons.
Journal
Anesthesiology
Author(s)
Krzisch M., Sultan S., Sandell J., Demeter K., Vutskits L., Toni N.
ISSN
1528-1175 (Electronic)
ISSN-L
0003-3022
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
118
Number
3
Pages
602-610
Language
english
Abstract
BACKGROUND: Adult neurogenesis occurs in the hippocampus of most mammals, including humans, and plays an important role in hippocampal-dependent learning. This process is highly regulated by neuronal activity and might therefore be vulnerable to anesthesia. In this article, the authors investigated this possibility by evaluating the impact of propofol anesthesia on mouse hippocampal neurons generated during adulthood, at two functionally distinct maturational stages of their development.
METHODS: Adult-born hippocampal neurons were identified using the cell proliferation marker bromodeoxyuridine or a retroviral vector expressing the green fluorescent protein in dividing cells and their progenies. Eleven or 17 days after the labeling procedure, animals (n = 3-5 animals per group) underwent a 6-h-long propofol anesthesia. Twenty-one days after labeling, the authors analyzed the survival, differentiation, and morphologic maturation of adult-born neurons using confocal microscopy.
RESULTS: Propofol impaired the survival and maturation of adult-born neurons in an age-dependent manner. Anesthesia induced a significant decrease in the survival of neurons that were 17 days old at the time of anesthesia, but not of neurons that were 11 days old. Similarly, propofol anesthesia significantly reduced the dendritic maturation of neurons generated 17 days before anesthesia, without interfering with the maturation of neurons generated 11 days before anesthesia.
CONCLUSIONS: These results reveal that propofol impairs the survival and maturation of adult-born hippocampal neurons in a developmental stage-dependent manner in mice.
Keywords
Anesthesia/adverse effects, Animals, Cell Aging/drug effects, Cell Survival/drug effects, Hippocampus/drug effects, Hippocampus/pathology, Male, Mice, Mice, Inbred C57BL, Neurogenesis/drug effects, Neurons/drug effects, Neurons/pathology, Propofol/toxicity
Pubmed
Web of science
Open Access
Yes
Create date
31/05/2013 12:46
Last modification date
20/08/2019 14:42
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