The hourglass model of evolutionary conservation during embryogenesis extends to developmental enhancers with signatures of positive selection.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_7EF03DE26E06
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The hourglass model of evolutionary conservation during embryogenesis extends to developmental enhancers with signatures of positive selection.
Journal
Genome research
Author(s)
Liu J., Viales R.R., Khoueiry P., Reddington J.P., Girardot C., Furlong EEM, Robinson-Rechavi M.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
09/2021
Peer-reviewed
Oui
Volume
31
Number
9
Pages
1573-1581
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Inter-species comparisons of both morphology and gene expression within a phylum have revealed a period in the middle of embryogenesis with more similarity between species compared with earlier and later time points. This "developmental hourglass" pattern has been observed in many phyla, yet the evolutionary constraints on gene expression, as well as the underlying mechanisms of how this is regulated, remain elusive. Moreover, the role of positive selection on gene regulation in the more diverged earlier and later stages of embryogenesis remains unknown. Here, using DNase-seq to identify regulatory regions in two distant Drosophila species (D. melanogaster and D. virilis), we assessed the evolutionary conservation and adaptive evolution of enhancers throughout multiple stages of embryogenesis. This revealed a higher proportion of conserved enhancers at the phylotypic period, providing a regulatory basis for the hourglass expression pattern. Using an in silico mutagenesis approach, we detect signatures of positive selection on developmental enhancers at early and late stages of embryogenesis, with a depletion at the phylotypic period, suggesting positive selection as one evolutionary mechanism underlying the hourglass pattern of animal evolution.
Keywords
Animals, Drosophila/genetics, Drosophila melanogaster/genetics, Embryonic Development/genetics, Evolution, Molecular, Gene Expression Regulation, Developmental, Regulatory Sequences, Nucleic Acid
Pubmed
Web of science
Open Access
Yes
Funding(s)
Swiss National Science Foundation / Projects / 173048
Create date
26/07/2021 9:44
Last modification date
20/07/2022 6:11
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