Measuring surface potential changes on leaves.

Details

Ressource 1Download: BIB_791806EB2CA7.P001.pdf (1974.30 [Ko])
State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_791806EB2CA7
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Measuring surface potential changes on leaves.
Journal
Nature Protocols
Author(s)
Mousavi S.A., Nguyen C.T., Farmer E.E., Kellenberger S.
ISSN
1750-2799 (Electronic)
ISSN-L
1750-2799
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
9
Number
8
Pages
1997-2004
Language
english
Abstract
We provide here a detailed protocol for studying the changes in electrical surface potential of leaves. This method has been developed over the years by plant physiologists and is currently used in different variants in many laboratories. The protocol records surface potential changes to measure long-distance electrical signals induced by diverse stimuli such as leaf wounding or current injection. This technique can be used to determine signaling speeds, to measure the connectivity between different plant organs and-by exploiting mutant plants-to identify transporters and ion channels involved in electrical signaling. The approach can be combined with the analysis of mRNA expression and of metabolite concentrations to correlate electrical signaling to specific physiological events. We describe how to use this protocol on Arabidopsis, looking at the effects of leaf wounding; however, it is broadly applicable to other plants and can be used to study other aspects of plant physiology. After wound infliction, surface potential recording takes ∼20 min per plant.
Pubmed
Web of science
Open Access
Yes
Create date
12/07/2014 8:51
Last modification date
20/10/2020 9:19
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