Kermit interacts with gαo, vang, and motor proteins in Drosophila planar cell polarity.

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Serval ID
serval:BIB_6F6942054721
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Kermit interacts with gαo, vang, and motor proteins in Drosophila planar cell polarity.
Journal
PLoS One
Author(s)
Lin C., Katanaev V.L.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2013
Volume
8
Number
10
Pages
e76885
Language
english
Abstract
In addition to the ubiquitous apical-basal polarity, epithelial cells are often polarized within the plane of the tissue - the phenomenon known as planar cell polarity (PCP). In Drosophila, manifestations of PCP are visible in the eye, wing, and cuticle. Several components of the PCP signaling have been characterized in flies and vertebrates, including the heterotrimeric Go protein. However, Go signaling partners in PCP remain largely unknown. Using a genetic screen we uncover Kermit, previously implicated in G protein and PCP signaling, as a novel binding partner of Go. Through pull-down and genetic interaction studies, we find that Kermit interacts with Go and another PCP component Vang, known to undergo intracellular relocalization during PCP establishment. We further demonstrate that the activity of Kermit in PCP differentially relies on the motor proteins: the microtubule-based dynein and kinesin motors and the actin-based myosin VI. Our results place Kermit as a potential transducer of Go, linking Vang with motor proteins for its delivery to dedicated cellular compartments during PCP establishment.
Pubmed
Web of science
Open Access
Yes
Create date
07/12/2013 16:51
Last modification date
20/10/2020 10:08
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