Functions of the <i>Dictyostelium</i> LIMP-2 and CD36 homologues in bacteria uptake, phagolysosome biogenesis and host cell defence.

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_68BE128564CF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Functions of the <i>Dictyostelium</i> LIMP-2 and CD36 homologues in bacteria uptake, phagolysosome biogenesis and host cell defence.
Journal
Journal of Cell Science
Author(s)
Sattler N., Bosmani C., Barisch C., Guého A., Gopaldass N., Dias M., Leuba F., Bruckert F., Cosson P., Soldati T.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
131
Number
17
Language
english
Abstract
Phagocytic cells take up, kill and digest microbes by a process called phagocytosis. To this end, these cells bind the particle, rearrange their actin cytoskeleton, and orchestrate transport of digestive factors to the particle-containing phagosome. The mammalian lysosomal membrane protein LIMP-2 (also known as SCARB2) and CD36, members of the class B of scavenger receptors, play a crucial role in lysosomal enzyme trafficking and uptake of mycobacteria, respectively, and generally in host cell defences against intracellular pathogens. Here, we show that the <i>Dictyostelium discoideum</i> LIMP-2 homologue LmpA regulates phagocytosis and phagolysosome biogenesis. The <i>lmpA</i> knockdown mutant is highly affected in actin-dependent processes, such as particle uptake, cellular spreading and motility. Additionally, the cells are severely impaired in phagosomal acidification and proteolysis, likely explaining the higher susceptibility to infection with the pathogenic bacterium <i>Mycobacterium marinum</i> , a close cousin of the human pathogen <i>Mycobacterium tuberculosis</i> Furthermore, we bring evidence that LmpB is a functional homologue of CD36 and specifically mediates uptake of mycobacteria. Altogether, these data indicate a role for LmpA and LmpB, ancestors of the family of which LIMP-2 and CD36 are members, in lysosome biogenesis and host cell defence.
Keywords
CD36, Dictyostelium discoideum, LIMP-2, Mycobacteria, Phagocytosis, Phagosome maturation
Pubmed
Web of science
Open Access
Yes
Create date
03/10/2018 13:09
Last modification date
20/08/2019 14:23
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