Peroxisome proliferator-activated receptor beta regulates acyl-CoA synthetase 2 in reaggregated rat brain cell cultures.

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Serval ID
serval:BIB_60E7401120E0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Peroxisome proliferator-activated receptor beta regulates acyl-CoA synthetase 2 in reaggregated rat brain cell cultures.
Journal
Journal of Biological Chemistry
Author(s)
Basu-Modak S., Braissant O., Escher P., Desvergne B., Honegger P., Wahli W.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
1999
Volume
274
Number
50
Pages
35881-35888
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that regulate the expression of many genes involved in lipid metabolism. The biological roles of PPARalpha and PPARgamma are relatively well understood, but little is known about the function of PPARbeta. To address this question, and because PPARbeta is expressed to a high level in the developing brain, we used reaggregated brain cell cultures prepared from dissociated fetal rat telencephalon as experimental model. In these primary cultures, the fetal cells initially form random aggregates, which progressively acquire a tissue-specific pattern resembling that of the brain. PPARs are differentially expressed in these aggregates, with PPARbeta being the prevalent isotype. PPARalpha is present at a very low level, and PPARgamma is absent. Cell type-specific expression analyses revealed that PPARbeta is ubiquitous and most abundant in some neurons, whereas PPARalpha is predominantly astrocytic. We chose acyl-CoA synthetases (ACSs) 1, 2, and 3 as potential target genes of PPARbeta and first analyzed their temporal and cell type-specific pattern. This analysis indicated that ACS2 and PPARbeta mRNAs have overlapping expression patterns, thus designating the ACS2 gene as a putative target of PPARbeta. Using a selective PPARbeta activator, we found that the ACS2 gene is transcriptionally regulated by PPARbeta, demonstrating a role for PPARbeta in brain lipid metabolism.
Keywords
Animals, Bezafibrate/pharmacology, Cell Aggregation, Cells, Cultured, Coenzyme A Ligases/genetics, Cycloheximide/pharmacology, DNA-Binding Proteins/metabolism, Dactinomycin/pharmacology, Embryo, Mammalian, Gene Expression Regulation/drug effects, Gene Expression Regulation, Enzymologic/drug effects, Genes, Reporter, Hela Cells, Humans, Isoenzymes/genetics, Kinetics, Neurons/cytology, Neurons/metabolism, Rats, Receptors, Cytoplasmic and Nuclear/genetics, Receptors, Cytoplasmic and Nuclear/physiology, Recombinant Fusion Proteins/biosynthesis, Telencephalon/cytology, Telencephalon/metabolism, Time Factors, Transcription Factors/genetics, Transcription Factors/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:11
Last modification date
20/08/2019 14:18
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