NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence.

Details

Ressource 1Download: 24308329_BIB_53BD1DDECAE6.pdf (849.75 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_53BD1DDECAE6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence.
Journal
Environmental Microbiology
Author(s)
Wenner N., Maes A., Cotado-Sampayo M., Lapouge K.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Publication state
Published
Issued date
2014
Volume
16
Number
4
Pages
1053-1068
Language
english
Abstract
The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non-coding RNA: NrsZ (nitrogen-regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post-transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Gene Expression Regulation, Bacterial, Glycolipids/metabolism, Nitrogen/metabolism, Pseudomonas aeruginosa/pathogenicity, Pseudomonas aeruginosa/physiology, RNA, Small Untranslated/chemistry, RNA, Small Untranslated/genetics, Virulence/genetics, Virulence Factors/genetics, beta-Galactosidase/genetics, beta-Galactosidase/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2014 11:05
Last modification date
20/08/2019 14:08
Usage data