Adverse effects of industrial multiwalled carbon nanotubes on human pulmonary cells

Details

Ressource 1Download: BIB_4FDAF0543B78.P001.pdf (8978.53 [Ko])
State: Public
Version: author
Secondary document(s)
Download: 19034795_Postprint.pdf (11047.80 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_4FDAF0543B78
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Adverse effects of industrial multiwalled carbon nanotubes on human pulmonary cells
Journal
Journal of Toxicology and Environmental Health. Part A
Author(s)
Tabet Lyes, Bussy Cyrill, Amara Nadia, Setyan Ari, Grodet Alain, Rossi Michel J., Pairon Jean-Claude, Boczkowski Jorge, Lanone Sophie
ISSN
1528-7394
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
72
Number
2
Pages
60-73
Language
english
Notes
SAPHIRID:77986
Abstract
The aim of this study was to evaluate adverse effects of multiwalled carbon nanotubes (MWCNT), produced for industrial purposes, on the human epithelial cell line A549. MWCNT were dispersed in dipalmitoyl lecithin (DPL), a component of pulmonary surfactant, and the effects of dispersion in DPL were compared to those in two other media: ethanol (EtOH) and phosphate-buffered saline (PBS). Effects of MWCNT were also compared to those of two asbestos fibers (chrysotile and crocidolite) and carbon black (CB) nanoparticles, not only in A549 cells but also in mesothelial cells (MeT5A human cell line), used as an asbestos-sensitive cell type. MWCNT formed agglomerates on top of both cell lines (surface area 15-35 μm2) that were significantly larger and more numerous in PBS than in EtOH and DPL. Whatever the dispersion media, incubation with 100 μg/ml MWCNT induced a similar decrease in metabolic activity without changing cell membrane permeability or apoptosis. Neither MWCNT cellular internalization nor oxidative stress was observed. In contrast, asbestos fibers penetrated into the cells, decreased metabolic activity but not cell membrane permeability, and increased apoptosis, without decreasing cell number. CB was internalized without any adverse effects. In conclusion, this study demonstrates that MWCNT produced for industrial purposes exert adverse effects without being internalized by human epithelial and mesothelial pulmonary cell lines. [Authors]
Keywords
Nanotubes, Carbon, Asbestos, Amphibole, Asbestos, Serpentine, Soot, Oxidative Stress, Pulmonary Alveoli, Epithelial Cells, Apoptosis
Pubmed
Web of science
Create date
19/01/2010 17:30
Last modification date
20/08/2019 14:05
Usage data