Stringent V beta requirement for the development of NK1.1+ T cell receptor-alpha/beta+ cells in mouse liver.

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License: CC BY-NC-SA 4.0
Serval ID
serval:BIB_4458
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stringent V beta requirement for the development of NK1.1+ T cell receptor-alpha/beta+ cells in mouse liver.
Journal
The Journal of experimental medicine
Author(s)
Ohteki T., MacDonald H.R.
ISSN
0022-1007
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
183
Number
3
Pages
1277-1282
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
The liver of C57BL/6 mice contains a major subset of CD4+8- and CD4-8- T cell receptor (TCR)-alpha/beta+ cells expressing the polymorphic natural killer NK1.1 surface marker. Liver NK1.1+TCR-alpha/beta+ (NK1+ T) cells require interaction with beta2-microglobulin-associated, major histocompatibility complex I-like molecules on hematopoietic cells for their development and have a TCR repertoire that is highly skewed to Vbeta8.2, Vbeta7, and Vbeta2. We show here that congenic C57BL/6.Vbeta(a) mice, which lack Vbeta8- expressing T cells owing to a genomic deletion at the Vbeta locus, maintain normal levels of liver NK1+ T cells owing to a dramatic increase in the proportion of cells expressing Vbeta7 and Vbeta2 (but not other Vbetas). Moreover, in C57BL/6 congenic TCR-V Vbeta3 and -Vbeta8.1 transgenic mice (which in theory should not express other Vbeta, owing to allelic exclusion at the TCR-beta locus), endogenous TCR-Vbeta8.2, Vbeta7, and Vbeta2 (but not other Vbetas) are frequently expressed on liver NK1+T cells but absent on lymph node T cells. Finally, when endogenous V beta expression is prevented in TCR-Vbeta3 and Vbeta8.1 transgenic mice (by introduction of a null allele at the C beta locus), the development of liver NK1+T cells is totally abrogated. Collectively, our data indicate that liver NK1+T cells have a stringent requirement for expression of TCR-Vbeta8.2, Vbeta7, or Vbeta2 for their development.
Keywords
Alleles, Animals, Antigens/biosynthesis, Antigens/genetics, Antigens, CD4/immunology, Antigens, Ly, Antigens, Surface, Flow Cytometry, Haplotypes, Histocompatibility Antigens Class I/immunology, Killer Cells, Natural/immunology, Lectins, C-Type, Liver/immunology, Lymph Nodes/immunology, Mice, Mice, Inbred Strains, Mice, Transgenic, NK Cell Lectin-Like Receptor Subfamily B, Organ Specificity, Polymorphism, Genetic, Protein Biosynthesis, Proteins/genetics, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/genetics, Sequence Deletion, T-Lymphocyte Subsets/immunology, beta 2-Microglobulin/immunology
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:40
Last modification date
20/08/2019 13:48
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