Immunosurveillance of lung melanoma metastasis in EBI-3-deficient mice mediated by CD8+ T cells.

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Serval ID
serval:BIB_22137C645067
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunosurveillance of lung melanoma metastasis in EBI-3-deficient mice mediated by CD8+ T cells.
Journal
Journal of Immunology
Author(s)
Sauer K.A., Maxeiner J.H., Karwot R., Scholtes P., Lehr H.A., Birkenbach M., Blumberg R.S., Finotto S.
ISSN
1550-6606
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
181
Number
9
Pages
6148-6157
Language
english
Abstract
EBV-induced gene 3 (EBI-3) codes for a soluble type I receptor homologous to the p40 subunit of IL-12 that is expressed by APCs following activation. In this study, we assessed the role of EBI-3 in a model of lung melanoma metastasis. Intravenous injection of the B16-F10 cell line resulted in a significant reduction of lung tumor metastasis in EBI-3(-/-) recipient mice compared with wild-type mice. The immunological finding accompanying this effect was the expansion of a newly described cell subset called IFN-gamma producing killer dendritic cells associated with CD8(+) T cell responses in the lung of EBI-3(-/-) mice including IFN-gamma release and TNF-alpha-induced programmed tumor cell death. Depletion of CD8(+) T cells as well as targeting T-bet abrogated the protective effects of EBI-3 deficiency on lung melanoma metastases. Finally, adoptive transfer of EBI-3(-/-) CD8(+) T cells into tumor bearing wild-type mice inhibited lung metastasis in recipient mice. Taken together, these data demonstrate that targeting EBI-3 leads to a T-bet-mediated antitumor CD8(+) T cell responses in the lung.
Keywords
Animals, CD8-Positive T-Lymphocytes, Cell Line, Transformed, Cell Line, Tumor, Cell Transformation, Neoplastic, Cytotoxicity, Immunologic, Gene Knockout Techniques, Immunologic Surveillance, Injections, Intravenous, Lung Neoplasms, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Mice, Knockout, Neoplasm Transplantation, Receptors, Cytokine, T-Box Domain Proteins
Pubmed
Web of science
Create date
12/12/2008 16:51
Last modification date
20/08/2019 12:59
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