EIU in the rat promotes the potential of syngeneic retinal cells injected into the vitreous cavity to induce PVR.

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Version: Final published version
Serval ID
serval:BIB_1966ECCD85DD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
EIU in the rat promotes the potential of syngeneic retinal cells injected into the vitreous cavity to induce PVR.
Journal
Investigative Ophthalmology and Visual Science
Author(s)
Behar-Cohen F.F., Thillaye-Goldenberg B., de Bizemont T., Savoldelli M., Chauvaud D., de Kozak Y.
ISSN
0146-0404 (Print)
ISSN-L
0146-0404
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
41
Number
12
Pages
3915-3924
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
PURPOSE: To determine whether syngeneic retinal cells injected in the vitreous cavity of the rat are able to initiate a proliferative process and whether the ocular inflammation induced in rats by lipopolysaccharide (LPS) promotes this proliferative vitreoretinopathy (PVR).
METHODS: Primary cultured differentiated retinal Müller glial (RMG) and retinal pigmented epithelial (RPE) cells isolated from 8 to 12 postnatal Lewis rats were injected into the vitreous cavity of 8- to 10-week-old Lewis rats (10(5) cells/eye in 2 microlieter sterile saline), with or without the systemic injection of 150 microgram LPS to cause endotoxin-induced uveitis (EIU). Control groups received an intravitreal injection of 2 microliter saline. At 5, 15, and 28 days after cell injections, PVR was clinically quantified, and immunohistochemistry for OX42, ED1, vimentin (VIM), glial fibrillary acidic protein (GFAP), and cytokeratin was performed.
RESULTS: The injection of RMG cells, alone or in combination with RPE cells, induced the preretinal proliferation of a GFAP-positive tissue, that was enhanced by the systemic injection of LPS. Indeed, when EIU was induced at the time of RMG cell injection into the vitreous cavity, the proliferation led to retinal folds and localized tractional detachments. In contrast, PVR enhanced the infiltration of inflammatory cells in the anterior segment of the eye.
CONCLUSIONS: In the rat, syngeneic retinal cells of glial origin induce PVR that is enhanced by the coinduction of EIU. In return, vitreoretinal glial proliferation enhanced the intensity and duration of EIU.
Keywords
Animals, Cell Transplantation, Cells, Cultured, Fluorescent Antibody Technique, Indirect, Glial Fibrillary Acidic Protein/metabolism, Injections, Keratins/metabolism, Lipopolysaccharides, Neuroglia/metabolism, Neuroglia/transplantation, Pigment Epithelium of Eye/metabolism, Pigment Epithelium of Eye/transplantation, Rats, Rats, Inbred Lew, Receptors, Complement 3b/metabolism, Retina/metabolism, Retina/transplantation, Retinal Detachment/etiology, Retinal Detachment/metabolism, Salmonella typhimurium, Transplantation, Isogeneic, Uveitis/complications, Uveitis/metabolism, Vimentin/metabolism, Vitreoretinopathy, Proliferative/etiology, Vitreoretinopathy, Proliferative/metabolism, Vitreous Body/surgery
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10/04/2014 9:19
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20/08/2019 12:50
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