CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.

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Version: Final published version
License: CC BY-NC-SA 4.0
Serval ID
serval:BIB_17100
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.
Journal
Journal of Cell Biology
Author(s)
Jantsch-Plunger V., Gönczy P., Romano A., Schnabel H., Hamill D., Schnabel R., Hyman A.A., Glotzer M.
ISSN
0021-9525
Publication state
Published
Issued date
2000
Volume
149
Number
7
Pages
1391-1404
Language
english
Notes
Publication types: Journal Article
Abstract
During cytokinesis of animal cells, the mitotic spindle plays at least two roles. Initially, the spindle positions the contractile ring. Subsequently, the central spindle, which is composed of microtubule bundles that form during anaphase, promotes a late step in cytokinesis. How the central spindle assembles and functions in cytokinesis is poorly understood. The cyk-4 gene has been identified by genetic analysis in Caenorhabditis elegans. Embryos from cyk-4(t1689ts) mutant hermaphrodites initiate, but fail to complete, cytokinesis. These embryos also fail to assemble the central spindle. We show that the cyk-4 gene encodes a GTPase activating protein (GAP) for Rho family GTPases. CYK-4 activates GTP hydrolysis by RhoA, Rac1, and Cdc42 in vitro. RNA-mediated interference of RhoA, Rac1, and Cdc42 indicates that only RhoA is essential for cytokinesis and, thus, RhoA is the likely target of CYK-4 GAP activity for cytokinesis. CYK-4 and a CYK-4:GFP fusion protein localize to the central spindle and persist at cell division remnants. CYK-4 localization is dependent on the kinesin-like protein ZEN-4/CeMKLP1 and vice versa. These data suggest that CYK-4 and ZEN-4/CeMKLP1 cooperate in central spindle assembly. Central spindle localization of CYK-4 could accelerate GTP hydrolysis by RhoA, thereby allowing contractile ring disassembly and completion of cytokinesis.
Keywords
Animals, Caenorhabditis elegans/cytology, Caenorhabditis elegans/genetics, Caenorhabditis elegans Proteins, Cell Division/physiology, Child, Cloning, Molecular, Embryo, Nonmammalian, Female, GTPase-Activating Proteins/genetics, GTPase-Activating Proteins/metabolism, Gene Expression Regulation, Developmental/physiology, Helminth Proteins/genetics, Helminth Proteins/metabolism, Humans, Kinesin/genetics, Kinesin/metabolism, Male, Mitotic Spindle Apparatus/physiology, Models, Biological, Mutation/physiology, Protein Structure, Tertiary/genetics, Subcellular Fractions/metabolism, rho GTP-Binding Proteins/genetics, rho GTP-Binding Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:10
Last modification date
20/08/2019 12:46
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