HIV-1 drug resistance transmission networks in southwest Switzerland.

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Serval ID
serval:BIB_07DD24ABA5AA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
HIV-1 drug resistance transmission networks in southwest Switzerland.
Journal
Aids Research and Human Retroviruses
Author(s)
Castro E., Khonkarly M., Ciuffreda D., Bürgisser P., Cavassini M., Yerly S., Pantaleo G., Bart P.A.
ISSN
1931-8405 ([electronic])
0889-2229 ([linking])
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
26
Number
11
Pages
1233-1238
Language
english
Abstract
To determine viral subtypes and resistance mutations to antiretroviral treatment (ART) in untreated HIV-1 acutely infected subjects from Southwest Switzerland. Clinical samples were obtained from the HIV primary infection cohort from Lausanne. Briefly, pol gene was amplified by nested PCR and sequenced to generate a 1?kb sequence spanning protease and reverse transcriptase key protein regions. Nucleotide sequences were used to assess viral genotype and ART resistance mutations. Blood specimens and medical information were obtained from 30 patients. Main viral subtypes corresponded to clade B, CRF02_AG, and F1. Resistant mutations to PIs consisted of L10V and accessory mutations 16E and 60E present in all F1 clades. The NNRTI major resistant mutation 103N was detected in all F1 viruses and in other 2 clades. Additionally, we identified F1 sequences from other 6 HIV infected and untreated individuals from Southwest Switzerland, harboring nucleotide motifs and resistance mutations to ART as observed in the F1 strains from the cohort. These data reveal a high transmission rate (16.6%) for NNRTI resistant mutation 103N in a cohort of HIV acute infection. Three of the 5 resistant strains were F1 clades closely related to other F1 isolates from HIV-1 infection untreated patients also coming from Southwest Switzerland. Overall, we provide strong evidence towards an HIV-1 resistant transmission network in Southwest Switzerland. These findings have relevant implications for the local molecular mapping of HIV-1 and future ART surveillance studies in the region.
Pubmed
Web of science
Open Access
Yes
Create date
15/12/2010 10:53
Last modification date
20/08/2019 12:30
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