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Functional Genomic mRNA Profiling of Colorectal Adenomas : Identification and Validation of CD44 and Splice Variant CD44v6 as Molecular Imaging Targets

Hartmans, Elmire; Orian-Rousseau, Veronique 1; Matzke-Ogi, Alexandra; Karrenbeld, Arend; Groot, Derk Jan A. de; Jong, Steven de; Dam, Gooitzen M. van; Fehrmann, Rudolf S. N.; Nagengast, Wouter B.
1 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)

Abstract:

Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide. High adenoma miss rates, especially seen in high-risk patients, demand for better endoscopic detection. By fluorescently 'highlighting' specific molecular characteristics, endoscopic molecular imaging has great potential to fulfill this need. To implement this technique effectively, target proteins that distinguish adenomas from normal tissue must be identified. In this study we applied in silico Functional Genomic mRNA (FGmRNA) profiling, which is a recently developed method that results in an enhanced view on the downstream effects of genomic alterations occurring in adenomas on gene expression levels. FGmRNA profiles of sporadic adenomas were compared to normal colon tissue to identify overexpressed genes. We validated the protein expression of the top identified genes, AXIN2, CEMIP, CD44 and JUN, in sporadic adenoma patient samples via immunohistochemistry (IHC). CD44 was identified as the most attractive target protein for imaging purposes and we proved its relevance in high-risk patients by demonstrating CD44 protein overexpression in Lynch lesions. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000068871
Originalveröffentlichung
DOI: 10.7150/thno.16816
Scopus
Zitationen: 11
Web of Science
Zitationen: 8
Dimensions
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2017
Sprache Englisch
Identifikator ISSN: 1838-7640
urn:nbn:de:swb:90-688713
KITopen-ID: 1000068871
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Theranostics
Verlag Ivyspring International Publisher
Band 7
Heft 2
Seiten 482-492
Vorab online veröffentlicht am 06.01.2017
Schlagwörter Functional Genomics, Cancer Genetics, Colorectal adenomas, Molecular Targeted Imaging, CD44 / CD44v6
Nachgewiesen in Web of Science
Dimensions
Scopus
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